DYCP-31DN Chisa 13/6 zitsime (1.0mm)

DYCP-31DN system ndi yopingasa electrophoresis system.Mu horizontal gel electrophoresis, gel osakaniza amaponyedwa molunjika ndikumizidwa mu buffer mkati mwa bokosi la gel. Bokosi la gel osakaniza limagawidwa m'zipinda ziwiri, ndi gel osakaniza agarose kuwalekanitsa awiriwo. Monga tanena kale, anode ili kumapeto kwina, pomwe cathode ili mbali inayo. Ionic kuthamanga buffer imalola kuti chowonjezera cha charger chipangidwe pakagwiritsidwa ntchito. Kuphatikiza apo, bufferyo imathandizira kuziziritsa gel osakaniza, omwe amawotcha ngati mtengo umayikidwa. Chotchinga chothamanga nthawi zambiri chimazunguliridwanso kuti aletse pH gradient kupanga. Tili ndi kukula kosiyana kwa zisa zoti tigwiritse ntchito. The zisa zosiyanasiyana zimapangitsa kuti yopingasa electrophoresis dongosolo limeneli kukhala yabwino kwa aliyense agarose gel osakaniza ntchito kuphatikizapo sitima zapamadzi electrophoresis, mofulumira electrophoresis ndi zochepa kuchuluka zitsanzo, DNA, sitima zapamadzi electrophoresis, pozindikira, kulekanitsa ndi kukonzekera DNA, ndi kuyeza kulemera kwa maselo.

Pa electrophoresis, gel osakaniza amapangidwa mu thireyi kuponyera. Thireyiyo ili ndi "zitsime" zazing'ono zomwe zimakhala ndi tinthu tating'onoting'ono tomwe timafuna kuyesa. Ma microliter angapo (µL) a yankho lomwe lili ndi tinthu ting'onoting'ono tomwe mukufuna kuyesa amalowetsedwa bwino m'zitsime. Kenako, chotchinga, chomwe chimayendetsa magetsi, chimatsanuliridwa muchipinda cha electrophoresis. Kenaka, thireyi yoponyera, yomwe ili ndi tinthu tating'ono, imayikidwa mosamala m'chipindamo ndikumizidwa mu buffer. Pomaliza, chipindacho chatsekedwa ndipo gwero lamagetsi limayatsidwa. Anode ndi cathode, zopangidwa ndi magetsi, zimakopa tinthu tating'ono totsutsana. Tinthu tating'onoting'ono timayenda pang'onopang'ono mu gel osakaniza kupita kumalo ena. Mphamvu imazimitsidwa, ndipo gel osakaniza amachotsedwa ndikuyang'aniridwa.