Electrophoresis Cell ya SDS-PAGE ndi Western Blot

Kufotokozera Kwachidule:

DYCZ-24DN ndi ya protein electrophoresis, pomwe DYCZ-40D ndiyosamutsa molekyulu ya protein kuchokera ku gel kupita ku nembanemba ngati nembanemba ya nitrocellulose pakuyesa kwa WesternBlot. Pano tili ndi kuphatikiza kwabwino kwa makasitomala athu omwe angakwaniritse ntchito yomwe experimenter angagwiritse ntchito thanki imodzi kuti achitegel electrophoresis, ndiyeno kusinthana gawo la elekitirodi kuchita kuyesa blotting ndi thanki yomweyo DYCZ-24DN. Zomwe mukufunikira ndi dongosolo la DYCZ-24DN chabe kuphatikiza gawo la DYCZ-40D Electrode lomwe limakupatsani mwayi wosinthira mwachangu komanso mosavuta kuchokera ku njira imodzi ya electrophoresis kupita ku ina.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera

kukula (L×W×H)	140 × 100 × 150mm
Kukula kwa Gel (L×W)	75 × 83 mm
Chisa	Zitsime 10 ndi zitsime 15
Makulidwe a Chisa	1.0mm ndi 1.5mm (Standard) 0.75mm (ngati mukufuna)
Chiwerengero cha Zitsanzo	20-30
Buffer Volume	400 ml
Kulemera	1kg

Kufotokozera

DYCZ-24DN ndi vertical electrophoresis cell (thanki / chipinda) kwa SDS-PAGE, Native PAGE etc. Mapuloteni electrophoresis. Seloli limatha kuponyera ndikuyendetsa gello pamalo amodzi. Ndizosavuta komanso zapadera zomwe ndizosavuta komanso zosavuta kutsitsa zitsanzo. Thankiyi imapangidwa ndi zinthu zapamwamba kwambiri za polycarbonate, zomwe zimakhala zolimba komanso zowonekera. Tanki yowonekera iyi imapangitsa kuti zikhale zosavuta kuyang'ana gel osakaniza mukayesa. DYCZ-24DN ili ndi maelekitirodi ochotsedwa omwe ndi osavuta kukonza. Ma elekitirodi amapangidwa ndi platinamu yoyera (≥99.95%) yomwe ndi electrolysis-corrosion komanso kupirira kutentha kwambiri.

Pambuyo pa gel electrophoresis, malinga ndi kufunikira koyesera, nthawi zina, woyesera amafunikira kusamutsa gel osakaniza ku chithandizo cholimba kuti awunikenso. Amatchedwa blotting experiment, yomwe ndi njira yosamutsira mapuloteni, DNA kapena RNA pa chonyamulira. Zimachitika pambuyo pa gel electrophoresis, kusamutsa mamolekyu kuchokera ku gel kupita ku nembanemba yotseka. Pambuyo pofufutika, mapuloteni omwe amasamutsidwa, DNA kapena RNA amawonetsedwa ndi madontho amtundu (mwachitsanzo, madontho a siliva a mapuloteni), mawonekedwe a autoradiography a mamolekyu opangidwa ndi radiolabelled (omwe amachitidwa chisanachitike), kapena zolemba zina za mapuloteni kapena nucleic acid. Zomalizazi zimachitika ndi ma antibodies kapena ma hybridization probes omwe amangomangiriza ku mamolekyu ena a blot ndikukhala ndi enzyme yolumikizana nawo. Pambuyo kutsuka koyenera, ntchito ya enzymatic iyi (ndiponso, mamolekyu omwe timafufuza mu blot) amawonetsedwa ndi ma incubation ndi mphamvu yoyenera, kutulutsa mtundu wakuda pa blot kapena chemiluminescent reaction yomwe imalembedwa ndi filimu yojambula.

Pa mphamvu yamagetsi ya cell yoyima ya gel electrophoresis, timalimbikitsa imodzi mwamagetsi owongolera timer yamtundu wa DYY-6C.

Kugwiritsa ntchito

Kwa SDS-PAGE, Native PAGE electrophoresis ndikusamutsa molekyulu ya protein kuchokera ku gel kupita ku nembanemba.

Mbali

Selo la DYCZ-24DN mini vertical gel electrophoresis cell la SDS-PAGE, Native PAGE electrophoresis ili ndi izi:

•Zopangidwa ndi polycarbonate yowoneka bwino kwambiri, zokongola komanso zolimba, zosavuta kuziwona;

• Pogwiritsa ntchito gel osakaniza pamalo oyambirira, amatha kuponyera ndi kuyendetsa gel osakaniza pamalo omwewo, osavuta komanso osavuta kupanga ma gels, ndikusunga nthawi yanu yamtengo wapatali;

• Mapangidwe apadera a wedge amatha kukonza chipinda cha gel olimba;

• Tanki yotchinga yokhala ndi maelekitirodi oyera a platinamu;

• Zosavuta komanso zosavuta kuwonjezera zitsanzo;

•Kukhoza rgel osakaniza limodzi kapena awiri nthawi imodzi;

• Sungani njira yosungira;

• Kukonzekera kwapadera kwa thanki kumapewa kuphulika ndi kutayikira kwa gel;

•Maelekitirodi ochotsedwa, osavuta kusamalira ndi kuyeretsa;

• Kuzimitsa-kuzimitsa pamene chivindikiro chatsegulidwa;

Ma electrode module, yomwe imatchedwanso Kuthandizira thupi kuti isamutsidwe kapena kusonkhana kwa elekitirodi ndi gawo lofunikira pakuchotsa dongosolo la DYCZ-40D. Amakhala ndi mbali zofiira ndi zakuda ndi maelekitirodi ofiira ndi akuda kuti awonetsetse kuti gel osakaniza akuyenda bwino panthawi yosamutsa, komanso kapangidwe kabwino kamene kamathandizira kuyika ndi kuchotsa makaseti osungira gel kuchokera ku bungwe lothandizira kuti asamutsidwe (electrode assembly).