Gene Electroporator GP-3000
Zofotokozera
| Chitsanzo | GP-3000 |
| Mawonekedwe a Pulse | Exponential Decay ndi Square Wave |
| Kutulutsa kwakukulu kwamagetsi | 401-3000V |
| Kutulutsa kwamagetsi otsika | 50-400V |
| High voltage capacitor | 10-60μF mu 1μF masitepe (10μF, 25μF, 35μF, 50μF, 60μF akulimbikitsidwa) |
| Low voltage capacitor | 25-1575μF mu 1μF masitepe (masitepe 25μF akulimbikitsidwa) |
| Parallel resistor | 100Ω-1650Ω mu 1Ω masitepe (50Ω akulimbikitsidwa) |
| Magetsi | 100-240VAC50/60HZ |
| Opareting'i sisitimu | Kuwongolera kwa Microcomputer |
| Nthawi yosasintha | ndi RC nthawi zonse, zosinthika |
| Kalemeredwe kake konse | 4.5kg |
| Makulidwe a Phukusi | 58x36x25cm |
Kufotokozera
Cell electroporation ndi njira yofunikira yopangira ma macromolecules akunja monga DNA, RNA, siRNA, mapuloteni, ndi mamolekyu ang'onoang'ono mkati mwa nembanemba zama cell.
Mothandizidwa ndi gawo lamphamvu lamagetsi kwakanthawi, nembanemba ya cell mu yankho imapeza mwayi wina. Nayitsa exogenous zinthu kulowa selo nembanemba m'njira yofanana electrophoresis. Chifukwa cha kukana kwakukulu kwa phospholipid bilayer ya nembanemba ya cell, ma bipolar voltages opangidwa ndi magetsi akunja amatengedwa ndi nembanemba ya cell, ndipo voteji yomwe imagawidwa mu cytoplasm imatha kunyalanyazidwa, pafupifupi palibe pano mu cytoplasm, moteronso kudziwa kawopsedwe kakang'ono mumayendedwe oyenera a electrophoresis.
Kugwiritsa ntchito
Angagwiritsidwe ntchito electroporation kusamutsa DNA mu maselo oyenerera, zomera ndi nyama maselo, ndi maselo yisiti. Monga electroporation ya mabakiteriya, yisiti, ndi tizilombo tating'onoting'ono tating'onoting'ono, kupatsirana kwa ma cell a mammalian, komanso kufalikira kwa minyewa ya zomera ndi ma protoplasts, kusakanizidwa kwa ma cell ndi kuphatikizika kwa jini, kuyambitsa kwa majini olembera ndikuwonetsa zolinga, kuyambitsa mankhwala, mapuloteni, ma antibodies, ndi mamolekyu ena kuti aphunzire momwe maselo amagwirira ntchito.
Mbali
• Kuchita bwino kwambiri: nthawi yochepa yotembenuka, kutembenuka kwakukulu, kubwerezabwereza;
• Kusungirako mwanzeru: kumatha kusunga magawo oyesera, osavuta kuti ogwiritsa ntchito agwiritse ntchito;
• Kuwongolera kolondola: kutulutsa kwamphamvu koyendetsedwa ndi microprocessor;Ø
• Kuwoneka kokongola: mapangidwe ophatikizika a makina onse, kuwonetsera mwachilengedwe, ntchito yosavuta.
FAQ
Q: Kodi Gene Electroporator ndi chiyani?
A: Gene Electroporator ndi chida chomwe chimagwiritsidwa ntchito poyambitsa ma genetic exogenous, monga DNA, RNA, ndi mapuloteni, m'maselo kudzera mu electroporation.
Q: Ndi maselo amtundu wanji omwe angayang'anitsidwe ndi Gene Electroporator?
A: Gene Electroporator ingagwiritsidwe ntchito poyambitsa ma genetic mumitundu yosiyanasiyana yama cell kuphatikiza mabakiteriya, yisiti, ma cell a zomera, ma cell a mammalian, ndi tizilombo tina.
Q: Kodi ntchito zazikulu za Gene Electroporator ndi ziti?
A:
• Electroporation ya mabakiteriya, yisiti, ndi tizilombo tina tating'ono: Kwa kusintha kwa majini ndi maphunziro a jini.
• Kusintha kwa ma cell a mammalian, minyewa ya zomera, ndi ma protoplasts: Kusanthula kwa ma jini, ma genomics ogwirira ntchito, ndi uinjiniya wa majini.
• Kuphatikizika kwa ma cell ndi ma gene fusion: Popanga ma hybrid cell ndikuyambitsa ma gene.
• Kuyambitsa ma jini a zolembera: Polemba ndi kutsata mafotokozedwe amtundu m'maselo.
• Kuyambitsa mankhwala, mapuloteni, ndi ma antibodies: Pofufuza momwe maselo amagwirira ntchito, kasamalidwe ka mankhwala, ndi kafukufuku wokhudzana ndi mapuloteni.
Q: Kodi Gene Electroporator imagwira ntchito bwanji?
A: Gene Electroporator imagwiritsa ntchito mphamvu yachidule yamagetsi yamagetsi kuti ipange pores osakhalitsa mu membrane ya cell, zomwe zimapangitsa kuti mamolekyu akunja alowe muselo. Nembanemba ya cell imayambiranso pambuyo pa kugunda kwamagetsi, ndikumanga mamolekyu omwe adalowetsedwa mkati mwa cell.
Q: Ubwino wogwiritsa ntchito Gene Electroporator ndi chiyani?
A: Kubwerezanso kwambiri komanso kuchita bwino, kugwiritsa ntchito mosavuta: Njira yosavuta komanso yachangu, kuwongolera kuchuluka, kusakhala ndi genotoxicity: Kuwonongeka pang'ono kwa chibadwa cha cell.
Q: Kodi Gene Electroporator ingagwiritsidwe ntchito pamitundu yonse yoyesera?
A: Ngakhale Gene Electroporator ndi yosinthasintha, mphamvu zake zimatha kusiyana malinga ndi mtundu wa selo ndi chibadwa chomwe chikuyambitsidwa. Ndikofunikira kukhathamiritsa mikhalidwe ya kuyesa kwina kulikonse.
Q: Ndi chisamaliro chapadera chiti chomwe chimafunika pambuyo poyambitsa maphunziro?
Yankho: Chisamaliro cha pambuyo poyambitsa chitha kuphatikizira kuyika ma cell m'malo ochira kuti awathandize kukonza ndikuyambiranso ntchito zabwinobwino. Zomwe zimapangidwira zimatha kusiyanasiyana malinga ndi mtundu wa cell komanso kuyesa.
Q: Kodi pali nkhawa zilizonse zokhudzana ndi chitetezo pogwiritsa ntchito Gene Electroporator?
A: Njira zodzitetezera ku labotale ziyenera kutsatiridwa. Gene Electroporator imagwiritsa ntchito magetsi okwera kwambiri, choncho kasamalidwe koyenera ndi njira zotetezera ziyenera kutsatiridwa kuti mupewe ngozi yamagetsi.
