DNA Gel Electrophoresis: Kusanthula Zidutswa Zamtundu

DNA gel electrophoresis ndi njira yodziwika bwino ya biology yomwe imagwiritsidwa ntchito polekanitsa ndi kusanthula zidutswa za DNA potengera kukula kwake. Kuchita zimenezi kumaphatikizapo kudzaza zidutswa za DNA za makulidwe osiyanasiyana pa gel opangidwa ndi agarose, chakudya chopezeka mu ndere zofiira.

Kukonzekera ndi kuponyera gel osakaniza agarose

1. Sungunulani agarose mu kuchuluka koyenera kwa electrophoresis buffer. Kuchuluka kwa gel osakaniza kumatsimikiziridwa ndi kuchuluka kwa kuchuluka kwa voliyumu, monga 1 g ya agarose mu 100.ml gel osakaniza ndi 1%.
2. Kutenthetsa chisakanizocho mu microwave, kuzungulira chidebe kuti mutsimikize kutha kwa agarose.
3. Onjezani ethidium bromide ku yankho la gel osakaniza mpaka 0,5mg/ ml. Ethidium bromide imalumikizana pakati pa mawiri awiri oyandikana ndi DNA ndikutulutsa fulorosisi ya lalanje pansi pa kuwala kwa UV. Dziwani kuti ethidium bromide ndi carcinogen, kotero kuigwira kumafuna njira zodzitetezera monga kuvala magolovesi.
4. Kuziziritsa gel osakaniza mu osamba m'madzi kuti thireyi ya gelsi isagwedezeke chifukwa cha kutentha kwambiri.
5. Ikani chisa mu gel osakaniza kuti mupange zitsanzo za zitsime. Sankhani zisa zoyenera kuchuluka kwa zitsanzo za DNA zomwe mukutsitsa. Thirani njira ya agarose gel mugel osakanizandi kulola kuti likhale lolimba kutentha.
6. Gelisiyo ikalimba, chotsani chisa. Ngati gel osagwiritsa ntchito nthawi yomweyo, kulungani ndi pulasitiki ndikusunga pa 4 ℃ mpaka pakufunika.

Kukonzekera ndi kuyendetsa gel osakaniza

Musanayambe electrophoresis, sakanizani chitsanzo cha DNA ndi buffer yotsegula. Chosungira chotsitsa nthawi zambiri chimakhala chokhazikika kasanu ndi kamodzi ndipo chimathandiza kuti chitsanzocho chiyimire pansi pa zitsime ndikutsata kayendedwe ka electrophoresis.

Khazikitsani magetsi ku voteji yomwe mwatchulidwa.

Onjezani chotchinga chokwanira cha electrophoresis ku tanki ya gel kuti mutseke pamwamba pa gel.Onetsetsanima elekitirodi olondola.

Kwezani zitsanzo za DNA ndi zolembera zolemetsa za mamolekyulu mu zitsime za gel.

Yatsani magetsi kuti muyambe electrophoresis.

Kuwona zidutswa za DNA zolekanitsidwa

Pambuyo pa electrophoresis, zimitsani magetsi ndikuchotsa gel osakaniza.

Gwiritsani ntchito gwero la kuwala kwa UV kuti muwunikire gel; Zidutswa za DNA zidzawoneka ngati magulu a lalanje a fulorosenti.

Lembani chithunzi cha gel kuti mufufuze zidutswa za DNA zolekanitsidwa.

Pambuyo poyesera, onetsetsani kuti gel osakaniza ndi electrophoresis atataya moyenera, potsatira malangizo a labotale. Valani magolovesi nthawi zonse pogwira ma gelisi ndi zotchingira zomwe zili ndi ethidium bromide kuti mutetezedwe ku zinthu zowopsazi.

DNA gel electrophoresis imagwiritsidwa ntchito kwambiri mu biology ya maselo ndi kafukufuku wa genetics poyesa kukula kwa mamolekyu a DNA, kulekanitsa zidutswa za DNA, kuzindikira masinthidwe amtundu, ndi zolemba zala za DNA, pakati pa ntchito zina. Ndi njira yosavuta komanso yothandiza yoyesera yomwe imathandizira kumvetsetsa kapangidwe ndi mawonekedwe a zitsanzo za DNA.

Beijing Liuyi Biotechnology Co.Ltd, wopanga katswiri yemwe amayang'ana kwambiri zinthu za electrophoresis zaka 50, amapereka matanki osiyanasiyana a electrophoresis (zipinda / ma cell) a gel electrophoresis, monga akasinja opingasa a electrophoresis (zipinda / ma cell)DNAgel electrophoresis, ndi akasinja ofukula electrophoresis (zipinda/maselo) a mapuloteni gel electrophoresis. Pakadali pano, imaperekanso mitundu yosiyanasiyana yamagetsi a electrophoresis, nduna yamdima yamabokosi osanthula ndi UV transilluminator. Mutha kusankha akasinja a electrophoresis a Beijing Liuyi (zipinda/maselo) kuti muyeze kulemera kwa mamolekyu a RNA ndikulekanitsa RNA yamitundu yosiyanasiyana ya labu yanu.

Apa titerolimbikitsamtundu umodzi wa yopingasa electrophoresis thanki (chipinda / selo) chitsanzoChithunzi cha DYCP-31DN kupanga ndi kuyendetsa gel osakaniza.

Mukatha kugwiritsa ntchito gel osakaniza, mutha kugwiritsa ntchito chithunzi chathu cha gel & kusanthula dongosoloWD-9413Bkuyang'ana, kusanthula ndi kujambula zithunzi za gel. Liuyi Biotech imaperekanso UV transilluminator (UV Analyzer) kuti muwone gel. Tili ndi mtundu wa bokosi lakuda la UV transilluminator (UV Analyzer).WD-9403A,9403C,WD-9403F, chotengera cha UV transilluminator (UV Analyzer).WD-9403Bndi m'manja UV transilluminator (UV Analyzer)WD-9403Epakuti mwasankha.

Mtundu wa Beijing Liuyi uli ndi mbiri yopitilira zaka 50 ku China ndipo kampaniyo imatha kupereka zinthu zokhazikika komanso zapamwamba padziko lonse lapansi. Kupyolera muzaka zachitukuko, ndizoyenera kusankha kwanu!

Tsopano tikuyang'ana mabwenzi, onse a OEM electrophoresis thanki ndi ogulitsa amalandiridwa.

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