Momwe Mungapangire DNA Electrophoresis mu Agarose Gel?

Apa tifotokoza momwe tingachitire agarose gel electrophoresis ndi wofufuza wathu mu labu ya Liuyi Biotech.

Kuyesera kusanachitike, tiyenera kuyang'ana zida, ma reagents, ndi zida zina zoyesera ndi zida zomwe timafunikira.

Kukonzekera zida zoyesera ndi zida

Zida za agarose gel electrophoresis

Selo yopingasa ya electrophoresis (thanki / chipinda), magetsi apakati ndi otsika a electrophoresis, chithunzi cha gel & dongosolo lowunikira.

Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) imapereka mitundu yosiyanasiyana yamaselo opingasa a electrophoresis (thanki/chipinda) ndi magetsi komanso makina osindikizira a gel. Zogulitsa zamtundu wa DYCP-31 ndi za electrophoresis, ndipo zopangira za DYY ndimagetsi a electrophoresis. Zogulitsa za WD-9413 ndizolemba za gel. Kuyambira ang'onoang'ono gel osakaniza kukula 60 × 60mm kuti gel osakaniza 250 × 250mm, tingathe kukwaniritsa zofunika zanu zosiyanasiyana kukula gel osakaniza. ChitsanzoDYCP-32Cakhoza kupanga gel osakaniza kufika kukula 250 × 250mm. TheDYY-6Cndi mphamvu zathu. Imathandizira kutulutsa kwa 400V, 400mA, 240W, chomwe ndi chinthu chathu chomwe chimagwiritsidwa ntchito ndi makasitomala athu. WD-9413BGel Image & Analysis System imagwiritsidwa ntchito posanthula ndi kufufuza ma gelisi, mafilimu, ndi ma blots pambuyo pa kuyesa kwa electrophoresis. Ndi chida chofunikira chokhala ndi gwero la kuwala kwa ultraviolet chowonera ndikujambula ma gels opaka utoto wa fulorosenti ngati ethidium bromide, komanso chowunikira choyera chowonera ndi kujambula ma gels opaka utoto ngati buluu wonyezimira wa coomassie.

Ma reagents a agarose gel electrophoresis

1. Sing'anga: gel osakaniza agarose

2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) ndi TBE (Tris-borate, EDTA, boric acid).

3.Chosungira chosungira: 6 × DNA Loading Buffer (Zapadera za DNA chitsanzo: EDAT, glycerine, Xylene cyanol ndi bromophenol blue)

The Dye

Utoto wa fulorosenti ngati EB, Gelred, goldview, GenGreen, GenView, SYBRGreen

TheConsumables

Malangizo oletsa kutsekereza (10μL), malangizo a pipette (200μL), malangizo a pipette (1000μL), 200μL\500μL\1.5ml centrifuge chubu.

DNA Marker

Kukonzekera molingana ndi kulemera kwa maselo kuti ayesedwe.

Gel ikuthamanga ndikuyang'ana

Choyamba, tiyenera kukonzekera gel osakaniza agarose. Kuchuluka kwa agarose mu gel kumatengera kukula kwa zidutswa za DNA kuti zisiyanitsidwe, ma gels ambiri amakhala pakati pa 0.5% -2%. Kutenga wathuChithunzi cha DYCP-31DNmwachitsanzo, Mutha kuponyera gel ang'onoang'ono, gel otakataka, gel otalikirapo ndi gel osakaniza molingana ndi zofunikira pakuyesa. Sankhani thireyi ya gel osakaniza yomwe mukufuna, ndikuyika chisa, kenako Thirani gel otenthetsera agarose mu chipangizo choponyera gel osakaniza.

Kenako, gel osakaniza akalimba, chotsani chisa mosamala komanso mofewa, kenako ikani tray ya gel mu thanki yotchinga. Thirani yankho la bafa mu thanki yotchingira ndikupangitsa kuti gel onse amizidwe muzotchingira. Kwezani zitsanzo mu zitsime ndi muyezo pipette. Gwirizanitsani ndiChithunzi cha DYCP-31DNndi magetsi a electrophoresis molondola, ndikuyika magawo oyendetsa gel osakaniza.

Onani m'maganizo mwanu zidutswa za DNA mu transilluminator ya UV.

Amutatha kugwiritsa ntchito gel osakaniza, mutha kugwiritsa ntchito chithunzi chathu cha gel & kusanthula dongosoloWD-9413Bkuyang'ana, kusanthula ndi kujambula zithunzi za gel. Liuyi Biotech imaperekanso UV transilluminator (UV Analyzer) kuti muwone gel. Tili ndi bokosi lakuda mtundu wa UV trAnsilluminator (UV Analyzer) chitsanzoWD-9403A, 9403C, WD-9403F, chotengera cha UV transilluminator (UV Analyzer).WD-9403Bndi m'manja UV transilluminator (UV Analyzer)WD-9403Epakuti mwasankha.

Chithunzi cha gel post electrophoresis

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