Mawu Oyamba
Gel electrophoresis ndi njira yofunikira mu biology ya maselo, yomwe imagwiritsidwa ntchito kwambiri pakulekanitsa mapuloteni, ma nucleic acid, ndi ma macromolecules ena. Kuwongolera koyenera kwa voliyumu yachitsanzo, mphamvu yamagetsi, ndi nthawi ya electrophoresis ndikofunikira kuti mupeze zotsatira zolondola komanso zobwerezeka.Mnzathu wa labotale akuperekanjira zabwino zoyendetsera magawowa pa SDS-PAGE gel electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis mankhwala
Voliyumu ya Zitsanzo: Kuonetsetsa Kusasinthasintha
Mukamachita electrophoresis ya SDS-PAGE, voliyumu yachitsanzo ndi chinthu chofunikira kwambiri chomwe chingakhudze kwambiri zotsatira zanu. Nthawi zambiri tikulimbikitsidwa kukweza 10 µL ya protein yonse pachitsime. Kuti muwonetsetse kusasinthika ndikupewa kufalikira kwa zitsanzo pakati pa zitsime zoyandikana, ndikofunikira kukweza voliyumu yofanana ya 1x yotsegula m'zitsime zilizonse zopanda kanthu. Kusamala kumeneku kumathandiza kupewa kufalikira kwa zitsanzo m’njira zoyandikana nazo, zomwe zingachitike ngati chitsimecho chikasiyidwa chopanda kanthu.
Musanakweze zitsanzo zanu, nthawi zonse yambani ndikuwonjezera cholembera cholemetsa pa chitsime chimodzi. Izi zimalola kuti azindikire mosavuta kukula kwa mapuloteni pambuyo pa electrophoresis.
Kuwongolera kwa Voltage: Kuwongolera Kuthamanga ndi Kukhazikika
Mphamvu yamagetsi yomwe imagwiritsidwa ntchito pa electrophoresis imakhudza mwachindunji liwiro lomwe zitsanzo zimasuntha kudzera mu gel osakaniza ndi kuthetsa kupatukana. Kwa SDS-PAGE, ndibwino kuti muyambe ndi magetsi otsika ozungulira 80V. Voltage yotsika iyi imalola zitsanzo kuti zisamuke pang'onopang'ono komanso molingana, ndikuziyika mugulu lakuthwa pamene zikulowa mu gel olekanitsa.
Zitsanzo zikalowa kwathunthu mu gel olekanitsa, voteji imatha kuwonjezeka mpaka 120V. Magetsi okwerawa amafulumizitsa kusamuka, kuwonetsetsa kuti mapuloteniwa amalekanitsidwa bwino malinga ndi kulemera kwawo kwa maselo. Ndikofunikira kuyang'anira momwe kutsogolo kwa utoto wa buluu wa bromophenol, zomwe zikuwonetsa kutha kwa electrophoresis. Kwa ma gels okhala ndi 10-12%, mphindi 80-90 ndizokwanira; Komabe, pa 15% gels, mungafunike kuwonjezera nthawi yothamanga pang'ono.
Kusamalira Nthawi: Kudziwa Nthawi Yoyimitsa
Kutenga nthawi ndi chinthu china chofunikira mu gel electrophoresis. Kuthamangitsa gel osakaniza kwa nthawi yayitali kwambiri kapena kwakanthawi kochepa kumatha kupangitsa kuti pakhale kupatukana kwakukulu. Kusamuka kwa utoto wa buluu wa bromophenol ndi chizindikiro chothandiza: ikafika pansi pa gel osakaniza, nthawi zambiri imakhala nthawi yoti asiye kuthamanga. Kwa gels wamba, monga 10-12%, nthawi ya electrophoresis ya mphindi 80-90 ndiyokwanira. Kwa ma gels apamwamba kwambiri, monga 15%, nthawi yothamanga iyenera kuwonjezedwa kuti zitsimikizire kulekanitsa kwathunthu kwa mapuloteni.
Kuwongolera kwa Buffer: Kugwiritsa Ntchitonso ndi Kukonzekera Zosungira
Electrophoresis buffer itha kugwiritsidwanso ntchito ka 1-2, kutengera momwe labotale yanu ilili. Komabe, kuti mupeze zotsatira zabwino, tikulimbikitsidwa kukonzekera 10x buffer yatsopano ndikuyichepetsa musanagwiritse ntchito. Izi zimatsimikizira kuti buffer imasunga mphamvu zake, zomwe zimapangitsa kuti pakhale zotsatira zodalirika za electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis mankhwala
Mwa kuwongolera mosamala kuchuluka kwachitsanzo, mphamvu yamagetsi, ndi nthawi ya electrophoresis, mutha kusintha kwambiri kulondola komanso kupangidwanso kwa zotsatira za gel electrophoresis yanu. Kugwiritsa ntchito njira zabwino izi pantchito yanu ya labotale kukuthandizani kuti mukwaniritse zomveka bwino komanso zodziwika bwino, zomwe zimatsogolera ku data yabwinoko yowunikira kutsika.
Ngati muli ndi njira zabwino zowonjezera kuyesa kwa gel electrophoresis, talandirani kuti mukambirane nafe!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Timaperekanso zida za labu monga PCR chida, vortex chosakanizira ndi centrifuge kwa labotale.
Ngati muli ndi pulani yogulira zinthu zathu, chonde musazengereze kulumikizana nafe. Mutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.
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Nthawi yotumiza: Aug-20-2024