Polyacrylamide imagwiritsidwa ntchito popanga ma cell biology ngati sing'anga ya electrophoresis ya mapuloteni ndi nucleic acid mu njira yotchedwa PAGE. Ndi mtundu wa zone electrophoresis njira ndi synthetics gel osakaniza otchedwa polyacrylamide monga kuthandiza sing'anga. Inamangidwa ndi S.Raymond ndi L.Weintraub mu 1959, ndipo kenako inalimbikitsidwa ndi kupangidwa ndi L.Ornstein ndi BJ Davis. Njirayi idagwiritsidwa ntchito kwambiri pambuyo pofotokozera komanso kusinthidwa ndi iwo mumalingaliro ndi njira zoyesera mu 1964.
Musanayambe ntchito polyacrylamide gel osakaniza electrophoresis, anthu makamaka ntchito pepala electrophoresis kwa zone EP. Koma pepalalo limakhala ngati sing'anga, lingokhala ndi ntchito yotsutsa-convection, palibe zotsatira zina zabwino. Ngakhale gel osakaniza a polyacrylamide sikuti ali ndi ntchito yotsutsa-convection komanso amatha kutenga nawo mbali pakulekanitsa kukuchitika mwachangu. Chifukwa polyacrylamide gel osakaniza ndi mtundu wa ukonde dongosolo, umene ndi polymerization ndi mtanda kulumikiza kuphatikiza acrylamide (Acr) ndi N, N-methylenebis (acrylamide). Acrylamide imatchedwa monomer, pamene N,N-methylenebis imatchedwa comonomer kapena crosslinker. Kupanga gel osakaniza ndi njira ya mankhwala polymerization. Kukula kwa pore kwa gel kumatha kuyendetsedwa, motero gel osakaniza amatha kupangidwa ndi magawo osiyanasiyana olumikizirana. Ngati pore kukula akuyandikira pafupifupi utali wozungulira wa molekyulu chitsanzo, kukana molekyulu kudutsa pore gel osakaniza adzakhala ndi ubale wapamtima ndi kukula ndi mawonekedwe a molekyulu pa electrophoresis. Chifukwa chake imapereka cholekanitsa chosinthika pakulekanitsa zidazo ndi zolipiritsa zofananira.
Pali njira ziwiri zomwe zimagwiritsidwa ntchito pa polyacrylamide gel electrophoresis, imodzi ndi disc electrophoresis, ndipo ina ndi slab electrophoresis. Ma slab electrophoresis adapangidwa mwapadera kuti azilekanitsa mapuloteni ndi DNA, ndipo nthawi zambiri, pali mitundu iwiri ya slab electrohporesis, yomwe ndi thanki yopingasa ya electrophoresis ndi thanki yoyima ya electrophoresis. Pamapuloteni, anthu amagwiritsa ntchito thanki yopingasa ya electrophoresis ya IFF ndi immunoelectrophoresis, apo ayi, anthu amagwiritsa ntchito akasinja oyimirira a electrophoresis kupanga mapuloteni.
Beijing Liuyi Biotechnology ili ndi mitundu yosiyanasiyana ya akasinja a slab electrophoresis a TSAMBA, kupatula kusanthula ndi kuzindikira zitsanzo zamapuloteni ndi polyacrylamide gel electrophoresis, itha kugwiritsidwanso ntchito kuyeza kulemera kwa maselo, kuyeretsa zitsanzo ndikukonzekera zitsanzo.
Tengani chitsanzoDYCZ-23Amwachitsanzo, yomwe ndi thanki yokhazikika ya electrophoresis ya Lab. Gwiritsani ntchito magalasi awiri kuti mupange chipinda cha gel kuti mupange gel, ndiyeno muwombe mbale zagalasi mwamphamvu kuti gel osakaniza asatayike. Kuchuluka kwa gel osakaniza kumadalira makulidwe a spacer. Nthawi zambiri, makulidwe ake ndi 1.5mm pakutulutsa bwino kutentha, komansoDYCZ-23Aimaperekanso 1.0mm makulidwe spacers poponya 1.0mm gel osakaniza. Kupatula thanki ya electrophoresis, kuti muthe kuyendetsa electrophoresis, magetsi amafunikiranso. Beijing Liuyi Biotechnology imapereka zosiyanasiyanaelectrophoresis magetsi. Kuchokera kumagetsi apamwamba mpaka kutsika kwamagetsi, mumasankha chitsanzo malinga ndi ntchito.
Mtundu wa Beijing Liuyi uli ndi mbiri yopitilira zaka 50 ku China ndipo kampaniyo imatha kupereka zinthu zokhazikika komanso zapamwamba padziko lonse lapansi. Kupyolera muzaka zachitukuko, ndizoyenera kusankha kwanu!
Tsopano tikuyang'ana mabwenzi, onse a OEM electrophoresis thanki ndi ogulitsa amalandiridwa.
Kuti mudziwe zambiri za ife, chonde titumizireni imelo[imelo yotetezedwa]kapena[imelo yotetezedwa].
Nthawi yotumiza: Oct-12-2022