Polymerase Chain Reaction (PCR) ndi Gel Electrophoresis: Njira Zofunikira mu Biology ya Molecular

Mu gawo lomwe likusintha nthawi zonse la biology ya ma molekyulu, Polymerase Chain Reaction (PCR) ndi Gel Electrophoresis zatuluka ngati njira zapangodya zomwe zimathandizira kuphunzira ndikusintha DNA. Njira izi sizongofunikira pakufufuza komanso zimagwira ntchito ponseponse muzofufuza, sayansi yazamalamulo, ndi biotechnology.

Chithunzi kuchokera patsamba la biology4alevel

PCR ndi njira yosinthira yopangidwa ndi Kary Mullis mu 1983, yomwe imalola asayansi kukulitsa gawo linalake la DNA mokulira. Njirayi imayamba ndi kufotokoza kwa DNA, kumene DNA yamitundu iwiri imatenthedwa mpaka 94 ° C, ndikupangitsa kuti igawike muzitsulo ziwiri. Izi zimatsatiridwa ndi annealing, pamene zoyamba—zotsatizana zazifupi za ma nucleotide—zimangiriza ku DNA ya chingwe chimodzi pa kutentha kochepa (nthawi zambiri pafupifupi 55°C). Pomaliza, gawo lokulitsa limapezeka pa 72 ° C, pomwe enzyme DNA polymerase imapanga chingwe chatsopano cha DNA powonjezera ma nucleotides ku zoyambira. Kuzungulira kumeneku kumabwerezedwa nthawi 20-40, zomwe zimatsogolera ku mamiliyoni a makope a DNA yomwe mukufuna.

Beijing LIUYI PCR Machine

DNA ikakulitsidwa, Gel Electrophoresis amagwiritsidwa ntchito kuti alekanitse ndi kusanthula zinthu za PCR. Njira imeneyi imaphatikizapo kusamuka kwa zidutswa za DNA kupyolera mu matrix a agarose gel mothandizidwa ndi magetsi. Mamolekyu a DNA amaperekedwa molakwika chifukwa cha msana wawo wa phosphate, ndipo amasamukira ku electrode yabwino. Gelisiyo imakhala ngati sieve, zomwe zimapangitsa kuti tiziduswa tating'ono ta DNA tisunthike mwachangu kuposa zazikulu. Zotsatira zake, zidutswa za DNA zimalekanitsidwa kutengera kukula kwake, ndi magulu apadera omwe amawonekera pansi pa kuwala kwa ultraviolet atadetsedwa ndi utoto ngati ethidium bromide.

Beijing LIUYIGel Electrophoresis Products

Kuphatikiza kwa PCR ndi Gel Electrophoresis ndikwamphamvu pamagwiritsidwe ambiri. Pofufuza zachipatala, PCR imagwiritsidwa ntchito pozindikira kupezeka kwa tizilombo toyambitsa matenda, kusintha kwa ma genetic, kapena ma DNA enieni okhudzana ndi matenda. Gel Electrophoresis ndiye imalola kuwonekera ndi kutsimikizira kwa zidutswa za DNA zokulitsa. Mu sayansi yazamalamulo, njirazi ndizofunikira pakusindikiza zala za DNA, komwe zimathandizira kufananiza zitsanzo za DNA kuchokera pazachiwembu ndi zomwe akuwakayikira.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Timaperekanso zida za labu monga PCR chida, vortex chosakanizira ndi centrifuge kwa labotale.

Beijing LIUYIGel Electrophoresis Products

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