Kukonzekera Gel Agarose Kwa Electrophoresis

Kukonzekera kwa Agarose Gel kwa Electrophoresis

Chidziwitso: Nthawi zonse valani magolovesi otayika!

Malangizo Pang'onopang'ono

Kulemera kwa Agarose Powder:use pepala loyezera ndi muyezo wamagetsi kuti muyeze 0.3g ya ufa wa agarose (potengera dongosolo la 30ml).

Kukonzekera TBST Buffer:pkonzani 30ml ya 1x TBST buffer mu 100ml Erlenmeyer botolo.

Kusungunula ufa wa Agarose:pathu ufa wa agarose mu TBST buffer ndikugwedezani bwino.Ikani iwo mkatiku microwave ndi kutentha (nthawi zambiri kwa masekondi 50, yokutidwa ndi zojambulazo za aluminiyamu) mpaka kusungunuka kwathunthu.

Kuzizira ndi Kuwonjezera Nuclease:use gloves kuchotsa kusakaniza mu microwave ndikusiya kuti kuziziritsa pang'ono m'madzi ozizira mpaka kutentha (pafupifupi 60 ° C). Onjezani 2µl wa nuclease (Eb m'malo) mukugwedeza kuti musakanize bwino.

Kukonzekera Gel Mold:

• CTatsamira ndi kupukutathireyi ya gel ndi chida choponyera gelmu tank electrophoresis.
•  Pgel osakanizathireyimu thanki yamkati ndikuyika chisacho pamalo okhazikika.
• Sakanizani njira ya agarose gel, itakhazikika mpaka 65 ° C, ndikutsanulira mosamalagel osakanizamuchipangizo choponyera gel, kufalitsa pang'onopang'ono mpaka kupanga gel osakaniza wosanjikiza pa mbale ya galasi.
• Lolani kuti ikhale kutentha kwapakati mpaka gel osakaniza atakhazikika kwathunthu.
• Chotsani zisa molunjika ndikuchotsa tepiyo.
• Ikani gel osakanizathireyimu thanki ya electrophoresis.

Zofunika: Onetsetsani kuti palibe thovu la mpweya pamalo a zisa. Pamwamba pa madzi ayenera kukhala yosalala popanda ripples.

Kuthamanga Gel

Kutsegula Gel

Gelisiyo ikalimba, ikani mu thanki ya electrophoresis ndikutsanulira chotchinga cha electrophoresis mpaka gel osakaniza amizidwa.

Kukonzekera Zitsanzo

• Tengani chikhomo ndikuyika bafa kuchokera mufiriji.
• Onjezani 6µl ya nkhokwe yotsegula ku zitsanzo ndikusakaniza bwino.
• Pogwiritsa ntchito micropipette, tsitsani pang'onopang'ono zitsanzozo m'zitsime zazikulu za gel (samalani kuti musabowole gel ndipo musatulutse voliyumu yonse kuti mupewe kuphulika kwa mpweya).
• Kwezani chikhomo mu zitsime zing'onozing'ono (kumbukirani malo ake).

Kuyambira Electrophoresis

• Phimbani thanki ya electrophoresis ndikuyamba electrophoresis mutangotsegula gel osakaniza.
• Ikani magetsi ku 60-100V. Zitsanzo zidzasamuka kuchokera ku electrode yolakwika (yakuda) kupita ku electrode yabwino (yofiira).
• Magetsi okwera amafupikitsa njira yolekanitsa ya gelisi ya agarose.
• Lekani electrophoresis pamene utoto wa buluu wa bromophenol ufika pafupifupi 1cm kuchokera m'mphepete mwa pansi pa mbale ya gel.

Kuwona Zotsatira

Pambuyo kulekanitsa magulu, siyani electrophoresis, chotsani gel osakaniza, ndi kuzindikira mwachindunji ndi kujambula.

Gwiritsani ntchito makina ojambulira ma gel ojambulira kujambula ndi kuyang'ana magulu (onani ngati pali magulu a chikhomo kapena zitsanzo).

Mukapeza mapu anu a gulu la gel, pezani cholembera. Kutengera chikhomo, mutha kudziwa magulu omwe mukufuna!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) akhala akupanga mankhwala a electrophoresis kwa zaka zoposa 50. Tikuwonetsa machitidwe athu opingasa a electrophoresis apagel osakanizaelectrophoresis.Sankhanimachitidwe osakanikirana a electrophoresiskuchokera ku Beijing liuyi pakuyesa kwanu kwa gel electrophoresis kuti akuthandizeni kufufuza kwanu mumakampani a biotechnology.

Timapereka mitundu yosiyanasiyana ya akasinja opingasa a electrophoresis oponya ndikuthamanga pang'onoagarosegels mpaka wamkuluagaroseangelo

Maupangiri Osankhira a Horizontal Immersed Gel Electrophoresis Unit

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Timaperekanso zida za labu monga PCR chida, vortex chosakanizira ndi centrifuge kwa labotale.

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