Chidule ChachidulezaAgarose Gel Electrophoresis
Agarose gel electrophoresis ndi njira yomwe imagwiritsidwa ntchito kwambiri mu biology ya molekyulu pakulekanitsa ma nucleic acid, monga DNA ndi RNA, kutengera kukula kwake. Njirayi imagwiritsa ntchito gel opangidwa kuchokera ku agarose, polysaccharide yachilengedwe yochokera ku udzu wam'nyanja. Gelisiyo imagwira ntchito ngati sieve ya molekyulu, yomwe imalola kuti mamolekyu asamuke kupyoleramo pamene magetsi akugwiritsidwa ntchito. Njira yolekanitsayi imathandiza ochita kafukufuku kusanthula ndikuwonetsa zitsanzo za nucleic acid powona magulu apadera omwe amapangidwa molingana ndi kukula kwake. Agarose gel electrophoresis ndi chida chofunikira kwambiri pantchito monga kusanthula zidutswa za DNA, kuwunika koyera, komanso kafukufuku wa biology yama cell.
Nthawi zambiri, thanki ya agarose gel electrophoresis imatchedwanso horizontal electrophoresis tank. Ntchito zazikulu za agarose gel electrophoresis ndi izi:
DNA Fragment Analysis:
Kulekanitsa ndi kusanthula zidutswa za DNA kutengera kukula, zofunika pa ntchito monga genotyping ndi DNA chala.
Kusanthula kwa RNA:
Kusamvana kwa mamolekyu a RNA, kuthandizira pakuphunzira kwa machitidwe a jini ndi kukhulupirika kwa RNA.
Kutsimikizira Kwazinthu za PCR:
Kutsimikizira kwa zinthu za polymerase chain reaction (PCR) kuti muwonetsetse kuti mukulitsidwa bwino ndikupeza makulidwe olondola a zidutswa.
Plasmid DNA Analysis:
Kuwunika kwa plasmid DNA pakuwongolera khalidwe, kutsimikizira zoyeserera za cloning, ndikuzindikiritsa DNA yophatikizanso.
Chiyerekezo Choyera:
Kutsimikiza kwa chiyero cha ma nucleic acid samples powonera zomwe zingaipitse kapena zonyansa.
Kafukufuku wa Molecular Biology:
Chida chofunikira m'ma laboratories a mamolekyulu a biology pazoyesera zosiyanasiyana, kuphatikiza kukonzekera kwa DNA ndikuletsa kugaya kwa ma enzyme.
Beijing Liuyi Biotechnology Co., Ltd. (yemwe kale inali Beijing Liuyi Instrument Factory) idakhazikitsidwa mu 1970. Ndi bizinesi yaukadaulo yomwe imagwira ntchito yopanga biochemistry ndi molekyulu ya biology ya labotale yowunikira zida. , ndipo ili ndi mzere wodalirika komanso wokwanira wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Tiuzeni zambiri za mtundu wake umodzi wa agarose gel electrophoresis-DYCP-31DN.
Chiyambi cha agel osakaniza electrophoresisthanki DYCP-31DN
Dimension (LxWxH) | 310 × 150 × 120mm |
Kukula kwa Gel (LxW) | 60x60mm, 60×120mm, 120×60mm, 120×120mm |
Chisa | 2+3 zitsime, zitsime 6+3, zitsime 8+18, ndi zitsime 11+25 |
Makulidwe a Chisa | 1.0mm, 1.5mm ndi 2.0mm |
Chiwerengero cha Zitsanzo | 2-100 |
Buffer Volume | 650 ml pa |
Kulemera | 1.0kg |
DYCP-31DN ndi thanki ya Liuyi ya agarose gel electrophoresis, yomwe ili ndi chipangizo choponyerapo choponyera ma gels 1-4. Itha kupanga gel osakaniza awiri kukula 60x60mm ndi gel osakaniza 120x60mm kapena gel osakaniza 60x120mm nthawi yomweyo. Gelisi yayikulu kwambiri yomwe imatha kuponyera ndi kukula kwa 120x120cm.
Themakulidwe a chisa operekedwandi1.0mm, 1.5mm ndi 2.0mm. Ndi kapangidwe ka electrode yochotseka, ndizosavuta kuyeretsa komanso kukonza. "Mapangidwe a mlatho" omwe cholinga chake ndi kuteteza njira yotchinga panthawi ya electrophoresis. Kugwiritsa ntchito moyenera ma buffer ndikofunikira pakusunga malo amagetsi okhazikika ndikuwonetsetsa kuti mamolekyu asiyanitsidwa molondola.
Zamtsogolo zina zofunika:
1) Zivundikiro ndi matupi akuluakulu a thanki (ma tanki a buffer) ndi owoneka bwino, owumbidwa, okongola, okhazikika, osindikizira abwino, osaipitsa ndi mankhwala;zosagwirizana ndi mankhwala, zosagwirizana ndi kuthamanga.
2) Ma elekitirodi amapangidwa ndi platinamu yoyera (chinthu choyera chachitsulo cholemekezeka ≥99.95%) chomwe chimakhala ndi kukana kwa dzimbiri kwa electroanalysis ndikupirira kutentha kwakukulu, ntchito yamagetsi yamagetsi ndiyabwino kwambiri.
3) Zitsime zachitsanzo nthawi zambiri zimakhala zovuta kuziwona, kotero gulu lakuda pa tray ya gel limapangitsa kuti zikhale zosavuta kuyang'ana ndikuyika zitsanzo.
4) Mphamvu imalephera mukatsegula chivindikiro kuti wosuta atetezeke.
Pali mitundu ingapo yamagetsi yoperekedwa kwa DYCP-31DN. Electrophoresis yotentha kwambiri magetsi is DYY-6C. Takulandilani kukaona tsamba lathu kuti mupeze mitundu yambiri yamagetsi.
Kuti muwone tiyegel osakaniza ndiDNA kapena RNA, Liuyi amapereka UV transilluminators kwa makasitomala.WD-9403C, WD-9403B ndiWD-9403X zonse zilipo kuti kasitomala asankhe.
Agarose gel electrophoresis ndi othandiza pa ntchito monga kuyang'ana kukula kwa zidutswa za DNA, kuyesa chiyero cha sampuli ya DNA, kapena kulekanitsa zidutswa za DNA kuti zifufuzidwe.
Ngati mukufuna kudziwa zambiri za mankhwalawa,chonde musazengereze kulumikizana nafe.Kutichezera ife ndiwww.gelepchina.com, or ymutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.
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Nthawi yotumiza: Jan-11-2024