Tidagawanapo nkhani zina zodziwika bwino zamagulu a electrophoresis m'mbuyomu, ndipo ife'Ndikufuna kugawana nawo zochitika zina zachilendo za polyacrylamide gel electrophoresis mbali ina. Ifemwachidule nkhani zimenezi kwa makasitomala athu' zowunikira kuti mupeze zifukwa ndi kukupeza zotsatira zolondola ndi kusintha khalidwe la kulekanitsa mapuloteni.
Gel osapangidwa ndi polymerized N'zotheka
Zifukwa ndi:
(a) Kusakwanira monomer chiyero, kufuna recrystallization.
(b) Mpweya wosungunuka mu gel osakaniza umalepheretsa gel polymerization. Pampu yogwira ntchito bwino iyenera kugwiritsidwa ntchito.
(c) Ammonium persulfate ndi yosagwira ntchito kapena yoperewera mu kuchuluka kwake. Konzani mwatsopano kapena gwiritsani ntchito gulu lina la ammonium sulfate lomwe lili ndi ndende yayikulu.
Tiye gel osakaniza kale polymerized popanda kuwonjezera madzi wosanjikiza.
Zifukwa ndi:
(a) Muziziziritsa gel osakaniza musanawonjezere chothandizira kuti muchepetse kuchuluka kwa ma polymerization.
(b) Chepetsani kuchuluka kwa TEMED kapena ammonium persulfate yogwiritsidwa ntchito.
(c) Kufulumizitsa ntchitoyo.
Gel imatuluka mu chipinda cha electrophoresis pambuyo polima
Izi zimachitika makamaka mu ma gels otsika kwambiri. Njira imodzi ndiyo kukulunga nembanemba ya dialysis pansi pa chubu kapena kugwiritsa ntchito porous polypropylene base.
Zitsanzo sizinapezeke pambuyo pa electrophoresis
Zifukwa ndi:
(a) Chiwerengero chosakwanira chachitsanzo. Onjezani kuchuluka kwachitsanzo.
(b) Kudetsa njira zothetsera kapena ndende kapena nthawi yodetsa ndizosakwanira. Bwezerani yankho lodetsa ndikuwonjezera ndende ndi nthawi yodetsa.
(c) Chitsanzocho chinayandama panthawi yotsegula. Wonjezerani kuchuluka kwa yankho lachitsanzo ndikugwirira ntchito mosamala.
(d) Kuyika kwa gel olekanitsa ndikokwera kwambiri, ndipo chitsanzocho sichinalowe mu gel. Sinthani ndende ya gel osakaniza moyenera.
(e) Kuphatikizika kwa gel olekanitsa ndikotsika kwambiri, ndipo chitsanzocho chatulutsidwa ndi gel olekanitsa. Kusintha ndende gel osakaniza ndi mulingo woyenera kwambiri electrophoresis zinthu.
(f) Ngati chitsanzocho ndi RNA, chikhoza kukhala ndi mapuloteni omwe amapanga zovuta zazikulu zomwe zimalepheretsa ma pores a gel. Chotsani bwino mapuloteni kuchokera ku chitsanzo cha RNA.
(g) Chitsanzocho chili ndi ma enzyme omwe amatulutsa hydrolyze chitsanzo, ndipo chitsanzocho chimasokoneza panthawi ya electrophoresis. Yeretsani bwino chitsanzo.
Beijing Liuyi Biotechnology Company Ltd imapanga zinthu zingapo zama electrophoresis zomwe zingathandize kuthana ndi zovuta zomwe mwina timakumana nazo pakuyesa kwathu kwa electrophoresis. Kampaniyo inakhazikitsidwa mu 1970. Inali ya dziko ndipo inapanga makina owotcherera amagetsi ndi mita ya mafakitale panthawiyo. Kuyambira 1979, Beijing Liuyi Biotechnology Company Ltd akuyamba kupanga mankhwala electrophoresis. Tsopano kampaniis m'modzi mwa otsogoleraopanga zida za labotale ndi zida zasayansi zozikidwa ku Beijing, China. Zake chizindikiro“LIUYI” ndi yotchuka ku China m'derali.
Zogulitsa za kampaniyi zimaphatikizapo zida zambiri za labotale, kuphatikiza yopingasa nucleic acid electrophoresis thanki, ofukula mapuloteni electrophoresis thanki/unit, black-boxmtundu UV analyzer,Gel Document Tracking Imaging Analyzer, ndi electrophoresis magetsi. Zogulitsazi zimagwiritsidwa ntchito kwambiri m'mabungwe ofufuza, mayunivesite, ndi mafakitale osiyanasiyana, monga mankhwala, biotechnology, ndi kuyang'anira chilengedwe.Kampaniyo ndi ISO9001 & ISO13485 kampani yotsimikizika ndipo ili ndi ziphaso za CE.
Palimitundu yosiyanasiyana yaofukulaakasinja electrophoresis kwamapuloteni electrophoresiskusanthula ndi kuzindikira zitsanzo zamapuloteni ndi polyacrylamide gel electrophoresis,ndikomanso kuyeza zitsanzo za kulemera kwa maselo, kuyeretsa zitsanzo ndi kukonzekera zitsanzo.Zogulitsa izi zonse zimalandilidwamsika wapakhomo ndi wakunja.
Mphamvu yamagetsi ya electrophoresis ndi gawo lofunikira laelectrophoresis dongosolo, kupereka gwero lokhazikika komanso lolondola la magetsi kuti ayendetse njira yolekanitsa.ItNthawi zambiri amapereka magetsi okhazikika kapena okhazikika ku dongosolo la electrophoresis, kutengera ndondomeko yoyesera. Zimathandizanso wogwiritsa ntchito kusintha mphamvu yamagetsi kapena zomwe zikuchitika panopa, komanso magawo ena monga nthawi ndi kutentha, kuti athetseretu mikhalidwe yolekanitsa pakuyesera kwina.
Fkapena kuyang'ana gel osakaniza, mutha kusankha UV Transilluminator WD-9403 mndandanda wopangidwa ndi Beijing Liuyi Biotechnology.A UV transilluminator ndi chida cha labotale chomwe chimagwiritsidwa ntchito powonera ndikusanthula DNA, RNA, ndi zitsanzo zama protein. Zimagwira ntchito powunikira zitsanzo ndi kuwala kwa UV, zomwe zimapangitsa kuti zitsanzozo ziwonekere ndikuwoneka. Pali mitundu ingapo ya UV transilluminatorzoperekedwa kwa inu ndi ife. WD-9403A ndiyowona makamaka poyang'ana protein electrophoresis, ndipo WD-9403F imagwiritsidwa ntchito powonera DNA ndi protein electrophoresis.
Zogulitsa izi zitha kukhala kuchokera pakuponyera gel mpaka kuyang'ana gel molingana ndi zomwe mumayesa.Tiuzeni zomwe mukufuna, OEM, ODM ndi ogulitsa amalandiridwa.We tidzayesetsa momwe tingathere kukupatsani katundu wathu ndi ntchito.
Ngati muli ndi pulani yogulira zinthu zathu, chonde musazengereze kulumikizana nafe. Mutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.
Nthawi yotumiza: May-23-2023