Protocol for Pre-cast Gel Protein Electrophoresis Experiment

Kukonzekera Kwachiyeso

Chongani Zida: Onetsetsani kuti chipinda cha protein electrophoresis, magetsi, ndi makina osinthira akugwira ntchito.TimaperekaChithunzi cha DYCZ-24DN kwa protein electrophoresis,Chithunzi cha DYCZ-40D kwa dongosolo kusamutsa, ndiDYY-6C kwa magetsi.

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Kukonzekera Zitsanzo: Konzani zitsanzo zanu molingana ndi kapangidwe kake. Tengani zitsanzo zamapuloteni ndi zochepetsera ndi ma protease ngati kuli kofunikira.

Konzani Electrophoresis Buffer: Tsatirani malangizo operekedwa ndi gel osakaniza kuti mukonzekere buffer ya electrophoresis pamalo oyenera.

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Kusamalira Gel Pre-cast:

Chotsani Pre-cast Gel: Tsegulani zoyikapo mosamala ndikuchotsa gel osakaniza mumtsuko wake, kusamala kuti musawononge matrix a gel.

Kuyika Zitsanzo: Kwezani zitsanzo zanu zomwe mwakonzekera muzitsime za gel pogwiritsa ntchito micropipette kapena zida zina zoyenera. Samalani ku dongosolo lotsegula ndi kuchuluka kwa chitsanzo chilichonse.

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Khazikitsani Electrophoresis Conditions: Khazikitsani mikhalidwe ya electrophoresis, kuphatikizapo mphamvu yamakono, magetsi, ndi nthawi. Onetsetsani kuti mwasankha mikhalidwe yoyenera kulekana bwino.

Kuthamanga kwa Electrophoresis

Yambitsani Electrophoresis: Ikani gel osakaniza mu chipinda cha electrophoresis, gwirizanitsani magetsi, ndikuyamba electrophoresis. Yang'anirani ndondomekoyi kuti muwonetsetse kuti madzi akuyenda bwino.

Complete Electrophoresis: Imitsani electrophoresis pamene zitsanzo zasamukira kumalo omwe mukufuna. Pewani kuyendetsa electrophoresis kwa nthawi yayitali kuti muteteze zitsanzo kuti zisathe gel osakaniza.

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Kusamutsa Mapuloteni

Konzani Transfer System: Chotsani mbale ya gel kuchokera mchipindamo ndikukonzekera kusamutsa mapuloteni. Izi zingaphatikizepo kudula nembanemba ndikukonzekera zosungira zosamutsa.

Sonkhanitsani Kukonzekera Kwakasinthidwe: Sonkhanitsani khwekhwe la kusamutsa mapuloteni molingana ndi malangizo operekedwa ndi njira yosinthira. Samalani ku dongosolo la msonkhano ndi kukhazikitsa mikhalidwe.

Thamangani Kutumiza Mapuloteni: Yambitsani njira yotumizira mapuloteni ndikuwunika momwe ikuyendera. Onetsetsani kuti nthawi yosinthira ndi zikhalidwe zikugwirizana ndi zomwe mukuyesa.

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Kukonza Pambuyo Posamutsa:

Kugwira Membrane: Sinthani nembanemba yomwe idasamutsidwa ngati pakufunika, yomwe ingaphatikizepo kudetsa, immunoblotting, kapena njira zina zosinthira pambuyo potengera zomwe mukufuna.

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Kuwunika kwa Zotsatira: Unikani zotsatira potengera momwe mumayesera ndikusinthira. Tanthauzirani zomwe mwapeza ndikupanga ma chart oyenera kapena ma graph.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.

Tsopano tikuyang'ana mabwenzi, onse a OEM electrophoresis thanki ndi ogulitsa amalandiridwa.

Ngati muli ndi pulani yogulira zinthu zathu, chonde musazengereze kulumikizana nafe. Mutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.

Chonde Jambulani nambala ya QR kuti muwonjezere pa WhatsApp kapena WeChat.

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Nthawi yotumiza: Dec-15-2023