Mtsogoleli Wam'mbali Pokonzekera Gel ya Agarose ya Electrophoresis

Kodi mumavutika kukonza gel osakaniza agarose?Tiyeni tizitsatirakatswiri wathu wa labu pokonzekera gel osakaniza.

Kukonzekera kwa gel osakaniza agarose kumaphatikizapo izi:

Kulemera kwa Agarose Powder

Yezerani kuchuluka kofunikira kwa ufa wa agarose molingana ndi zomwe mukufuna pakuyesa kwanu. Kuchuluka kwa agarose kumayambira 0.5% mpaka 3%. Kuyika kwapamwamba kumagwiritsidwa ntchito kulekanitsa mamolekyu ang'onoang'ono a DNA, pamene otsika kwambiri ndi mamolekyu akuluakulu.

Kukonzekera Buffer Solution

Onjezani ufa wa agarose ku chotchinga choyenera cha electrophoresis, monga 1 × TAE kapena 1 × TBE. Kuchuluka kwa buffer kuyenera kufanana ndi kuchuluka kwa gel ofunikira pakuyesa kwanu.

Kuchotsa Agarose

Kutenthetsa chisakanizo cha agarose ndi buffer mpaka agarose itasungunuka kwathunthu. Izi zitha kuchitika pogwiritsa ntchito microwave kapena mbale yotentha. Sakanizani njirayo pang'onopang'ono kuti isatenthedwe. Njira ya agarose iyenera kumveka bwino popanda tinthu tating'onoting'ono.

Kuzizira kwa Agarose Solution

Lolani kuti madzi otentha a agarose azizire mpaka 50-60 ° C. Sakanizani yankho panthawi yozizira kuti mupewe kulimba msanga.

Kuwonjezera Madontho a Nucleic Acid (Mwasankha)

Ngati mukufuna kuwona DNA kapena RNA mu gel, mukhoza kuwonjezera banga la nucleic acid, monga GelRed kapena ethidium bromide, panthawiyi. Pogwira madonthowa, valani magolovesi ndikusamala, chifukwa amatha kukhala oopsa.

Kuponya Gel

Thirani utakhazikika njira ya agarose mu okonzeka electrophoresis gel osakaniza nkhungu. Ikani chisa kuti mupange zitsime zachitsanzo, kuwonetsetsa kuti zisa ndi zotetezedwa ndipo yankho lagawidwa mofanana mu nkhungu.

 Gel Solidification

Lolani gel osakaniza kuti akhazikike kutentha kwa firiji, zomwe nthawi zambiri zimatenga mphindi 20-30 kutengera kuchuluka kwake komanso makulidwe ake.

Rkutulutsa Chisa

Gelisiyo ikalimba, chotsani chisa mosamala kuti muwone zitsime zachitsanzo. Ikani gel osakaniza pamodzi ndi nkhungu mu chipinda cha electrophoresis ndikuchiphimba ndi kuchuluka koyenera kwa electrophoresis buffer, kuonetsetsa kuti gel osakaniza amizidwa kwathunthu.

Kukonzekera kwa Electrophoresis

Gelisi ikakonzeka, tsitsani zitsanzo zanu m'zitsime, ndipo pitirizani kuyesa electrophoresis.

Ngati muli ndi vuto lililonse ndikukonzekera gel osakaniza, omasuka kulankhula nafe. Tili ndi akatswiri a labu omwe akupezeka kuti azilumikizana nanu.

Ndife okondwa kugawana nawo nkhani zabwino: thanki yathu yotchuka ya DYCP-31DN yopingasa ya electrophoresis pano ikukwezedwa., tithandizeni kuti mudziwe zambiri tsopano!

DYCP-31DN yopingasa electrophoresis thanki

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Timaperekanso zida za labu monga PCR chida, vortex chosakanizira ndi centrifuge kwa labotale.

Ngati muli ndi pulani yogulira zinthu zathu, chonde musazengereze kulumikizana nafe. Mutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.

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