Kodi njira yotenthetsera njinga mu PCR ndi yotani?

Polymerase Chain Reaction (PCR) ndi njira ya biology ya mamolekyulu yomwe imagwiritsidwa ntchito kukulitsa zidutswa za DNA. Itha kuonedwa ngati mawonekedwe apadera a DNA kufananitsa kunja kwa chamoyo. Chofunikira chachikulu cha PCR ndikutha kuchulukitsa kuchuluka kwa DNA.

Chidule cha Polymerase Chain Reaction Cycle

Chithunzi chochokera ku Bioninja

Kodi njira yotenthetsera njinga mu PCR ndi yotani?

PCR ili ndi njira zitatu zofunika:

1. Kusintha kwa Template DNA: The template DNA imatenthedwa kufika ku 93°C kwa nthawi inayake, kuchititsa kuti DNA yazingwe ziwiri igawike kukhala chingwe chimodzi, kuikonzekera kuti ikhale yoyambira mumzere wotsatira.

2. Annealing of Template DNA and Primers: Pambuyo pa denaturation, kutentha kumatsitsidwa kufika pafupifupi 55 ° C, kulola zoyambira kumangirira kumayendedwe owonjezera pa template ya chingwe chimodzi cha DNA.

3. Kuwonjezera kwa Primers: Pa 72 ° C, DNA template-primer complex ikupita patsogolo mothandizidwa ndi DNA polymerase (monga Taq DNA polymerase), pogwiritsa ntchito dNTPs monga ma substrates kuti apange chingwe chatsopano chothandizira pogwiritsa ntchito template ya DNA.

Beijing LIUYI PCR Machine

Mwa kubwereza ma denaturation, annealing, and extensions, “semi-conservative replication strands” zambiri zimapangidwa, zomwe zitha kukhala ngati ma tempulo a mikombero yotsatira. Kuzungulira kulikonse kumatenga pafupifupi mphindi 2 mpaka 4, zomwe zimalola kuti jini yomwe mukufuna kuti ichuluke nthawi zambiri mkati mwa maola awiri kapena atatu.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) yakhala ikupanga zida za electrophoresis kwa zaka zopitilira 50 ndi gulu lathu laukadaulo laukadaulo ndi R&D pakati. Tili ndi mzere wodalirika komanso wathunthu wopanga kuchokera pamapangidwe mpaka kukayendera, ndi nyumba yosungiramo zinthu, komanso chithandizo chamalonda. Zogulitsa zathu zazikulu ndi Electrophoresis Cell (thanki / chipinda), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. Timaperekanso zida za labu monga PCR chida, vortex chosakanizira ndi centrifuge kwa labotale.

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