PCR Thermal Cycler WD-9402M
Zofotokozera
| Chitsanzo | WD-9402M |
| Mphamvu | 96 × 0.2ml |
| Chubu | 96x0.2ml (PCR mbale popanda/semi siketi), 12x8x0.2ml n'kupanga, 8x12x0.2ml n'kupanga, 0.2ml machubu (kutalika 20 ~ 23mm) |
| Block Temperature Range | 0-105 ℃ |
| Kuletsa Kulondola kwa Kutentha | ± 0.2 ℃ |
| Block Kutentha Uniformity | ± 0.5℃ |
| Mtengo Wotenthetsera (avareji) | 4 ℃ |
| Mlingo Wozizira (avareji) | 3 ℃ |
| Kuwongolera Kutentha | Block/Tube |
| Mtengo wa Gradient. Mtundu | 30-105 ℃ |
| Kutentha kwa Max | 5℃/s |
| Max.Kuzizira Kwambiri 4.5℃ /S | 4.5 ℃/s |
| Gradient Set Span | Max. 42 ℃ |
| Kulondola kwa Kutentha kwa Gradient | ± 0.3 ℃ |
| Kutentha kowonetsera kulondola | 0.1 ℃ |
| Kutentha Lid Kutentha osiyanasiyana | 30 ℃ ~ 110 ℃ |
| Chivundikiro Chotenthetsera Chokha | Zimitseni zokha ngati chitsanzo chatsika kuposa 30 ℃ kapena pulogalamu yatha |
| Kuchulukitsa Nthawi / Kuchepa | -599~599 S kwa Long PCR |
| Kutentha Kuwonjezeka / Kuchepa | -9.9 ~ 9.9 ℃ kwa Touchdown PCR |
| Chowerengera nthawi | 1s~59min59sec/ Zopanda malire |
| Mapulogalamu osungidwa | 10000+ |
| Max.Cycles | 99 |
| Max.Masitepe | 30 |
| Imani Ntchito | Inde |
| Touchdown Ntchito | Inde |
| Ntchito yayitali ya PCR | Inde |
| Chiyankhulo | Chingerezi |
| Kuyimitsa Pulogalamu Ntchito | Inde |
| 16 ℃ Ntchito Yogwira Kutentha | Zopanda malire |
| Zochitika zenizeni zenizeni | Zithunzi-zolemba zikuwonetsedwa |
| Kulankhulana | USB 2.0 |
| Makulidwe | 200mm × 300mm×170mm (W×D×H) |
| Kulemera | 4.5kg |
| Magetsi | 100-240VAC , 50/60Hz, 600W |
Kufotokozera
Thermal cycler imagwira ntchito potenthetsa mobwerezabwereza ndikuziziritsa zomwe zili ndi DNA kapena RNA template, primers, ndi nucleotides. Kutentha kwapang'onopang'ono kumawongoleredwa bwino kuti mukwaniritse zofunikira, kuwongolera, ndi kukulitsa njira za PCR.
Nthawi zambiri, woyendetsa matenthedwe amakhala ndi chipika chokhala ndi zitsime zingapo kapena machubu pomwe chosakanizacho chimayikidwa, ndipo kutentha kwa chitsime chilichonse kumayendetsedwa palokha. Chotchingacho chimatenthedwa ndikuzizidwa pogwiritsa ntchito chinthu cha Peltier kapena makina ena otentha ndi ozizira.
Ma cyclers ambiri otentha amakhala ndi mawonekedwe osavuta kugwiritsa ntchito omwe amalola wogwiritsa ntchito kukonza ndikusintha magawo apanjinga, monga kutentha kwa annealing, nthawi yowonjezera, ndi kuchuluka kwa ma cycle. Athanso kukhala ndi chiwonetsero chowonera momwe zimachitikira, ndipo mitundu ina imatha kupereka zinthu zapamwamba monga kuwongolera kutentha kwa gradient, masinthidwe angapo a block, ndi kuyang'anira ndi kuyang'anira kutali.
Kugwiritsa ntchito
Polymerase Chain Reaction (PCR) ndi njira ya biology yomwe imagwiritsidwa ntchito kwambiri pazinthu zosiyanasiyana. Ntchito zina zodziwika bwino za PCR ndi:
DNA Amplification: Cholinga chachikulu cha PCR ndikukulitsa ma DNA omwe adatsata. Izi ndizofunikira kuti mupeze DNA yokwanira kuti muwunikenso kapena kuyesa.
Kuyeza kwa Genetic: PCR imagwiritsidwa ntchito kwambiri poyesa chibadwa kuti azindikire zolembera kapena masinthidwe okhudzana ndi matenda. Ndikofunikira pazifukwa zodziwira matenda komanso kuphunzira za chibadwa.
DNA Cloning: PCR imagwiritsidwa ntchito kupanga chigawo chachikulu cha DNA, chomwe chimatha kupangidwa kukhala vekitala kuti ipitirire kapena kusanthula.
Kusanthula kwa Forensic DNA: PCR ndiyofunikira mu sayansi yazamalamulo pakukulitsa zitsanzo za miniti za DNA zopezedwa pamilandu. Zimathandizira kuzindikira anthu komanso kukhazikitsa maubwenzi obadwa nawo.
Kuzindikira kwa tizilombo tating'onoting'ono: PCR imagwiritsidwa ntchito pozindikira tizilombo toyambitsa matenda mu zitsanzo zachipatala kapena zitsanzo zachilengedwe. Amalola kuti adziwike mwachangu za matenda opatsirana.
Quantitative PCR (qPCR kapena Real-Time PCR): qPCR imathandizira kuwerengetsa kwa DNA panthawi yokulitsa. Amagwiritsidwa ntchito poyeza milingo ya ma jini, kuzindikira kuchuluka kwa ma virus, ndikuwunika kuchuluka kwa ma DNA omwe adatsatizana.
Maphunziro a Chisinthiko cha Molecular: PCR imagwiritsidwa ntchito pofufuza kusiyana kwa majini pakati pa anthu, maubwenzi osinthika, ndi kusanthula kwa phylogenetic.
Environmental DNA (eDNA) Kusanthula: PCR imagwiritsidwa ntchito kuti izindikire kukhalapo kwa zamoyo zinazake mu zitsanzo zachilengedwe, zomwe zimathandizira pazachilengedwe komanso maphunziro achilengedwe.
Genetic Engineering: PCR ndi chida chofunikira kwambiri pakupanga ma genetic kuti adziwitse ma DNA angapo mu zamoyo. Amagwiritsidwa ntchito popanga ma genetically modified organisms (GMOs).
Kutsata Kukonzekera kwa Laibulale: PCR ikutenga nawo gawo pakukonza malaibulale a DNA aukadaulo wotsatizana wa mibadwo yotsatira. Zimathandizira kukulitsa zidutswa za DNA pazotsatira zotsatizana.
Site-Directed Mutagenesis: PCR imagwiritsidwa ntchito poyambitsa masinthidwe enaake mumayendedwe a DNA, kulola ofufuza kuti aphunzire zotsatira za kusintha kwa majini.
DNA Fingerprinting: PCR imagwiritsidwa ntchito mu njira zolembera zala za DNA pozindikiritsa munthu payekha, kuyezetsa abambo, ndikukhazikitsa maubale achilengedwe.
Mbali
•Kuwoneka kokongola, kukula kophatikizika, komanso mawonekedwe olimba.
•Zokhala ndi chowotcha chapamwamba, chabata cha axial-flow kuti chizigwira ntchito mwakachetechete.
• Imakhala ndi magwiridwe antchito ambiri a 30 ℃, kulola kukhathamiritsa kwa zoyeserera kuti zikwaniritse zofunikira zoyeserera.
•5-inch high-definition color-definition color touchscreen for intuitive and easy operation, amathandizira kusintha kosavuta, kupulumutsa, ndi kuyendetsa mapulogalamu.
• Makina ogwiritsira ntchito mafakitale, omwe amathandizira mosalekeza komanso opanda zolakwika 7x24.
•Kusamutsa deta mwachangu kupita ku USB flash drive kuti musunge zosunga zobwezeretsera pulogalamu, kukulitsa mphamvu yosungira.
• Ukadaulo wapamwamba kwambiri wozizira wa semiconductor ndi ukadaulo wapadera wowongolera kutentha kwa PID umakweza magwiridwe antchito mpaka kutalika kwatsopano: kuwongolera kutentha kwapamwamba, kutentha kwachangu ndi kuzizira, komanso kutentha kwa module yofananira.
FAQ
Q: Kodi woyendetsa matenthedwe ndi chiyani?
Yankho: Makina oyendetsa matenthedwe ndi chipangizo cha labotale chomwe chimagwiritsidwa ntchito kukulitsa ma DNA kapena ma RNA kudzera mu polymerase chain reaction (PCR). Zimagwira ntchito poyendetsa njinga kudzera mukusintha kwa kutentha, zomwe zimapangitsa kuti ma DNA atsatidwe kuti akwezedwe.
Q: Ndi zigawo ziti zazikulu za woyendetsa matenthedwe?
A: Zigawo zazikulu za cycler matenthedwe zimaphatikizapo chipika chotenthetsera, chozizira cha thermoelectric, masensa kutentha, microprocessor, ndi gulu lowongolera.
Q: Kodi chozungulira chotenthetsera chimagwira ntchito bwanji?
Yankho: Woyendetsa matenthedwe amagwira ntchito potenthetsa ndi kuziziritsa zitsanzo za DNA motsatizana za kutentha. Kupalasa njinga kumaphatikizapo ma denaturation, annealing, ndi masitepe owonjezera, chilichonse chimakhala ndi kutentha kwake komanso nthawi yake. Zozungulira izi zimalola kuti ma DNA awonjezeke kudzera mu polymerase chain reaction (PCR).
Q: Ndi zinthu ziti zofunika kuziganizira posankha choyendetsa matenthedwe?
Yankho: Zina zofunika kuziganizira posankha choyendetsa chotenthetsera ndi monga kuchuluka kwa zitsime kapena machubu ochitira, kuchuluka kwa kutentha ndi liwiro la panjira, kulondola komanso kufanana kwa kutentha, komanso mawonekedwe a wogwiritsa ntchito ndi kuthekera kwa mapulogalamu.
Q: Kodi mumasunga bwanji chozungulira chotenthetsera?
Yankho: Kuti musunge chozungulira chotenthetsera, ndikofunikira kuyeretsa chotchingira chotenthetsera nthawi zonse ndi machubu ochitirapo kanthu, kuyang'ana kung'ambika ndi kung'ambika pazigawo zake, ndikuwongolera masensa a kutentha kuti muwonetsetse kutentha kolondola komanso kosasintha. Ndikofunikiranso kutsatira malangizo a wopanga pokonza ndi kukonza nthawi zonse.
Q: Ndi njira ziti zomwe zimakonda kuthetsera vuto kwa woyendetsa matenthedwe?
Yankho: Njira zina zothanirana ndi vuto la woyendetsa matenthedwe ndi monga kuyang'ana zida zotayirira kapena zowonongeka, kutsimikizira kutentha koyenera ndi nthawi, ndikuyesa machubu kapena mbale zomwe zawonongeka kapena kuwonongeka. Ndikofunikiranso kutchula malangizo a wopanga kuti mupeze njira zothetsera mavuto ndi zothetsera.
