Pulsed Field Gel Electrophoresis CHEF Mapper A7
Zofotokozera
| Chitsanzo | CHEF Mapper A7 |
| Mphamvu yamagetsi yamagetsi | 0.5V/cm mpaka 9.6V/cm, kukwezedwa ndi 0.1V/cm |
| Maximum Current | 0.5A |
| Maximum Voltage | 350V |
| Pulse Angle | 0-360 ° |
| Time Gradient | Linear ndi sanali mzere |
| Kusintha Nthawi | 50ms mpaka 18h |
| Nthawi Yothamanga Kwambiri | 999h ku |
| Nambala ya Electrodes | 24, yoyendetsedwa paokha |
| Multi-State Vector Change | Imathandizira mpaka ma vector 10 pa pulse cycle |
| Kutentha Kusiyanasiyana | 0 ℃ mpaka 50 ℃, vuto kuzindikira <± 0.5 ℃ |
Kufotokozera
Pulsed field gel electrophoresis (PFGE) imalekanitsa mamolekyu a DNA posintha gawo lamagetsi pakati pa ma elekitirodi osiyanasiyana omwe ali ndi malo, zomwe zimapangitsa kuti mamolekyu a DNA, omwe amatha kukhala mamiliyoni awiriawiri oyambira kuti abwererenso ndikusuntha kudzera pores agarose gel pa liwiro losiyana. Imakwaniritsa kusamvana kwakukulu mkati mwamtunduwu ndipo imagwiritsidwa ntchito makamaka mu biology yopanga; kuzindikira mibadwo yachilengedwe ndi tizilombo tating'onoting'ono; kafukufuku wa epidemiology ya maselo; maphunziro a zidutswa zazikulu za plasmid; kutanthauzira kwa chibadwa cha matenda; kupanga mapu amtundu wa majini, kusanthula kwa RFLP, ndi zolemba zala za DNA; kafukufuku wa imfa ya maselo; maphunziro okhudza kuwonongeka ndi kukonza kwa DNA; kudzipatula ndi kusanthula genomic DNA; kulekana kwa chromosomal DNA; kumanga, kuzindikira, ndi kusanthula malaibulale akuluakulu a magulu akuluakulu; ndi transgenic research.t ndende zotsika ngati 0.5 ng/µL (dsDNA).
Kugwiritsa ntchito
Zoyenera kuzindikira ndikulekanitsa mamolekyu a DNA kuyambira 100bp mpaka 10Mb kukula, kukwaniritsa kusamvana kwakukulu mkati mwamtunduwu.
Mbali
• Ukadaulo Wapamwamba: Zimaphatikiza matekinoloje a CHEF ndi PACE pulsed-field kuti akwaniritse zotsatira zabwino kwambiri ndi njira zowongoka, zosapindika.
• Ulamuliro Wodziimira: Umakhala ndi ma elekitirodi a platinamu 24 odzilamulira okha (0.5mm m'mimba mwake), ndi electrode iliyonse yosinthidwa payekha.
• Ntchito Yowerengera Mwadzidzidzi: Imaphatikiza zosintha zingapo zazikulu monga ma voltage gradient, kutentha, kusintha kolowera, nthawi yoyambira, nthawi yomaliza, nthawi yosinthira pano, nthawi yonse yothamanga, voteji, ndi zamakono zowerengera zokha, kuthandiza ogwiritsa ntchito kukwaniritsa zoyeserera bwino.
• Unique Algorithm: Imagwiritsa ntchito njira yapadera yoyendetsera mphamvu ya pulse kuti ikhale ndi zotsatira zabwino zolekanitsa, kusiyanitsa mosavuta pakati pa DNA yozungulira ndi yozungulira, ndi kulekanitsa kwapadera kwa DNA yaikulu yozungulira.
• Kusungirako Pulogalamu: Imasunga mpaka mapulogalamu a 15 ovuta kuyesa, omwe ali ndi ma modules osachepera 8.
• Multi-State Vector Change: Imathandizira mpaka ma vector 10 pa pulse cycle, kulola kutanthauzira kwa ngodya iliyonse, magetsi, ndi nthawi.
• Transition Slope: Linear, concave, or convex pogwiritsa ntchito hyperbolic function.
• Kukonzekera: Kulemba ndi kuyambiranso electrophoresis ngati dongosolo lasokonezedwa chifukwa cha kulephera kwa mphamvu.
• Zosasinthika: Zimalola ogwiritsa ntchito kukhazikitsa zomwe akufuna.
• Kusinthasintha: Dongosololi limatha kusankha ma voltage gradient ndi nthawi zosinthira pamitundu ina ya kukula kwa DNA.
• Chophimba Chachikulu: Chokhala ndi chophimba cha 7-inch LCD kuti chizigwira ntchito mosavuta, chokhala ndi mapulogalamu apadera a mapulogalamu kuti agwiritse ntchito mosavuta komanso mosavuta.
• Kuzindikira Kutentha: Zofufuza zapawiri za kutentha zimazindikira mwachindunji kutentha kwa bafa ndi malire olakwika osakwana ± 0.5℃.
• Njira Yozungulira: Imabwera ndi makina ozungulira a buffer omwe amayendetsa bwino ndikuyang'anira kutentha kwa yankho la buffer, kuonetsetsa kutentha kosalekeza ndi ionic balance panthawi ya electrophoresis.
• Chitetezo Chapamwamba: Kumaphatikizapo chivundikiro chachitetezo cha acrylic chowonekera chomwe chimazimitsa mphamvu mukachikweza, komanso ntchito zoteteza mochulukira komanso zopanda katundu.
• Kusintha Kosinthika: Tanki ya electrophoresis ndi gel caster imakhala ndi mapazi osinthika kuti azitha kuwongolera.
• Kupanga Mold: Tanki ya electrophoresis imapangidwa ndi mawonekedwe osakanikirana a nkhungu popanda kugwirizana; choyikapo ma elekitirodi chimakhala ndi ma elekitirodi a platinamu a 0.5mm, kuwonetsetsa kukhazikika komanso zotsatira zoyeserera zokhazikika.
• Pulse angle: Mpweya wothamanga ukhoza kusankhidwa mwaufulu pakati pa 0-360 °, kulola ogwiritsa ntchito kukwaniritsa kulekanitsa koyenera kuyambira chromosomal yaikulu mpaka DNA yaing'ono ya plasmid mkati mwa dongosolo lomwelo..
• Pulse Time Gradient: Zimaphatikizapo mizere yozungulira komanso yopanda mzere (convex ndi concave) pulse time gradients. Ma gradients osagwirizana ndi mizere amapereka mwayi wosiyana wosiyana, womwe umalola ogwiritsa ntchito kudziwa bwino kukula kwa zidutswa.
• Kuwunika Nthawi Yeniyeni: Pa nthawi imodzimodziyo amawonetsa magawo okhazikitsidwa ndi momwe amagwirira ntchito, ogwirizana ndi pulogalamu yowunikira nthawi yeniyeni.
• Ziphuphu Zachiwiri: Ukadaulo wachiwiri wa pulse ukhoza kufulumizitsa kutulutsidwa kwa DNA kuchokera ku gel agarose, kuthandizira kulekanitsa zidutswa zazikulu kwambiri za DNA ndikuwongolera kusintha.
• Yogwirizana ndi PulseNet China: Dongosololi limatha kulumikizana ndi netiweki yapadziko lonse lapansi yowunikira tizilombo toyambitsa matenda ndi PulseNet China network monitoring network, kulola kusiyanitsa zidutswa zokhala ndi mamolekyu ofanana.
FAQ
Q: Kodi Pulsed Field Gel Electrophoresis ndi chiyani?
A: Pulsed Field Gel Electrophoresis ndi njira yomwe imagwiritsidwa ntchito polekanitsa mamolekyu akuluakulu a DNA potengera kukula kwake. Zimaphatikizapo kusinthana kolowera kwa gawo la magetsi mu matrix a gel kuti athe kulekanitsa zidutswa za DNA zomwe ndi zazikulu kwambiri kuti zithe kuthetsedwa ndi chikhalidwe cha agarose gel electrophoresis.
Q: Kodi ntchito za Pulsed Field Gel Electrophoresis ndi ziti?
A: Pulsed Field Gel Electrophoresis imagwiritsidwa ntchito kwambiri mu biology ya maselo ndi chibadwa cha:
• Kupanga mapu a mamolekyu akuluakulu a DNA, monga ma chromosome ndi ma plasmid.
• Kudziwa kukula kwa ma genome.
• Kuphunzira kusintha kwa majini ndi maubwenzi osinthika.
• Molecular epidemiology, makamaka potsata miliri ya matenda opatsirana.
• Kusanthula kuwonongeka kwa DNA ndi kukonza.
• Kuwona kukhalapo kwa majini kapena ma DNA otsatizana.
Q: Kodi Pulsed Field Gel Electrophoresis imagwira ntchito bwanji?
Yankho: Pulsed Field Gel Electrophoresis imagwira ntchito poyika mamolekyu a DNA kumalo amagetsi omwe amasinthasintha. Izi zimalola mamolekyu akuluakulu a DNA kuti adziwongoleranso pakati pa ma pulse, kuwapangitsa kuyenda kwawo kudzera mu matrix a gel. Mamolekyu ang'onoang'ono a DNA amayenda mofulumira kwambiri kudzera mu gel, pamene zazikulu zimayenda pang'onopang'ono, zomwe zimalola kupatukana kwawo malinga ndi kukula kwake.
Q: Kodi mfundo kumbuyo Pulsed Field Gel Electrophoresis ndi chiyani?
A: Pulsed Field Gel Electrophoresis imalekanitsa mamolekyu a DNA kutengera kukula kwake poyang'anira nthawi ndi njira ya mphamvu zamagetsi. Munda wosinthirawu umapangitsa kuti mamolekyu akulu a DNA azidziwongolera mosalekeza, zomwe zimapangitsa kuti asamuke kudzera mu matrix a gel ndikupatukana molingana ndi kukula.
Q: Kodi ubwino wa Pulsed Field Gel Electrophoresis ndi chiyani?
A: Kusamvana kwakukulu pakulekanitsa mamolekyu akuluakulu a DNA mpaka mamiliyoni angapo.Kutha kuthetsa ndi kusiyanitsa zidutswa za DNA za kukula kwake.Kusinthasintha pogwiritsira ntchito, kuchokera ku microbial typing kupita ku genetics ya molecular ndi genomics.Njira yokhazikitsidwa ya maphunziro a epidemiological ndi genetic mapu.
Q: Ndi zida ziti zomwe zimafunikira Pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis imafuna chipangizo cha electrophoresis chokhala ndi maelekitirodi apadera kuti apange minda yothamanga. Agarose gel matrix okhala ndi ndende yoyenera komanso buffer. Mphamvu yamagetsi yomwe imatha kupanga ma pulses apamwamba kwambiri.Kuzizira kwapang'onopang'ono kutulutsa kutentha komwe kumapangidwa panthawi ya electrophoresis, ndi mpope wozungulira.
