Electrophoresis Transfer All-in-one System
Kufotokozera
| Mafotokozedwe a Electrophoresis Tank | |
| Kukula kwa Gel (LxW) | 83 × 73 mm |
| Chisa | 10 zitsime (Standard) 15 zitsime (ngati mukufuna) |
| Makulidwe a Chisa | 1.0 mm (Wamba) 0.75, 1.5 mm (Njira) |
| Chipinda Chachidule cha Glass | 101 × 73 mm |
| Spacer Glass Plate | 101 × 82 mm |
| Buffer Volume | 300 ml |
| Mafotokozedwe a Transfer Module | |
| Malo Otsekera (LxW) | 100 × 75 mm |
| Chiwerengero cha Ma Gel Holders | 2 |
| Distance ya Electrode | 4cm pa |
| Buffer Volume | 1200 ml |
| Mafotokozedwe a Electrophoresis Power Supply | |
| Dimension (LxWxH) | 315 x 290 x 128 mm |
| Kutulutsa kwa Voltage | 6-600V |
| Zotulutsa Panopa | 4-400mA |
| Mphamvu Zotulutsa | 240W |
| Malo Otulutsa | 4 awiriawiri mofanana |
Kufotokozera
Electrophoresis kusamutsa dongosolo lonse-mu-limodzi lili ndi thanki ya electrophoresis yokhala ndi chivindikiro, magetsi okhala ndi gulu lowongolera, ndi gawo losinthira ndi ma electrode. Tanki ya electrophoresis imagwiritsidwa ntchito kuponyera ndikuyendetsa ma gels, ndipo gawo losinthira limagwiritsidwa ntchito kunyamula gel ndi sangweji ya membrane panthawi yosinthira, ndipo imakhala ndi bokosi lozizira kuti lisatenthedwe. Mphamvu yamagetsi imapereka mphamvu yamagetsi yofunikira kuyendetsa gel osakaniza ndikuyendetsa kusamutsa kwa mamolekyu kuchokera ku gel kupita ku nembanemba, ndipo imakhala ndi gulu lowongolera logwiritsa ntchito pokhazikitsa ma electrophoresis ndikusintha zinthu. Njira yosinthira imaphatikizapo maelekitirodi omwe amayikidwa mu thanki ndikukhudzana ndi gel ndi nembanemba, ndikumaliza gawo lamagetsi lomwe limafunikira kusamutsa.
The electrophoresis transfer all-in-one system ndi chida chofunikira kwa ofufuza ndi akatswiri omwe amagwira ntchito ndi zitsanzo za mapuloteni. Mapangidwe ake ophatikizika komanso kugwiritsa ntchito kwake kosavuta kumapangitsa kuti ikhale yowonjezera ku labotale iliyonse yomwe imakhudzidwa ndi kafukufuku wamamolekyulu kapena kafukufuku wa biochemistry.
Kugwiritsa ntchito
Electrophoresis transfer all-in-one system ndi chida chofunikira kwambiri pazasayansi yama cell biology, makamaka pakuwunika kwa mapuloteni. Mapuloteni omwe amasamutsidwa amazindikiridwa pogwiritsa ntchito ma antibodies enieni munjira yotchedwa Western blotting. Njirayi imalola ochita kafukufuku kuzindikira mapuloteni enieni omwe amawakonda ndikuwerengera momwe amafotokozera.
Zowonetsedwa
• Mankhwalaikuyenera kukula kochepa PAGE gel electrophoresis;
•Zogulitsa's magawo, zowonjezera zimagwirizana kwathunthu ndi zinthu zazikulu pamsika;
•Kapangidwe kapamwamba ndi kamangidwe kofewa;
• Onetsetsani zotsatira zabwino zoyesera kuchokera ku kuponyera gel osakaniza mpaka kuthamanga kwa gel osakaniza;
•Mofulumira kusamutsa ang'onoang'ono kukula gel osakaniza;
• Makaseti awiri okhala ndi Gel amatha kuikidwa mu thanki;
•Itha kuthamanga mpaka ma gelisi awiri mu ola limodzi. Ikhoza kugwira ntchito usiku wonse kuti isamutsidwe kwambiri;
•Makaseti okhala ndi ma gel okhala ndi mitundu yosiyanasiyana amatsimikizira kuyika bwino.
FAQ
Q: Kodi electrophoresis transfer all-in-one system imagwiritsidwa ntchito bwanji?
A: Njira ya electrophoresis kusamutsa zonse-mu-imodzi imagwiritsidwa ntchito kusamutsa mapuloteni kuchokera ku gelisi ya polyacrylamide kupita ku nembanemba kuti iwunikenso, monga Western blotting.
Q: Ndi kukula kwa gel osakaniza komwe kungapangidwe ndikusamutsidwa pogwiritsa ntchito electrophoresis kusamutsa dongosolo lonse mumodzi?
A: electrophoresis kutengerapo zonse mu umodzi dongosolo akhoza kuponyera ndi kuthamanga gel osakaniza kukula 83X73cm pa dzanja kuponyera, ndi 86X68cm chisanadze kuponyera gel osakaniza. Malo osinthira ndi 100X75cm.
Q: Kodi The electrophoresis kusamutsa zonse mu umodzi dongosolo ntchito?
A: Electrophoresis transfer all-in-one system imagwiritsa ntchito electrophoresis kusamutsa mapuloteni kuchokera ku gel kupita ku nembanemba. Mapuloteni amayamba kulekanitsidwa ndi kukula pogwiritsa ntchito polyacrylamide gel electrophoresis (PAGE) kenako amasamutsidwa ku nembanemba pogwiritsa ntchito magetsi.
Q: Ndi mitundu yanji ya nembanemba yomwe ingagwiritsidwe ntchito ndi electrophoresis kusamutsa dongosolo lonse-mu-limodzi?
A: Mitundu yosiyanasiyana ya nembanemba ingagwiritsidwe ntchito ndi electrophoresis kusamutsa dongosolo lonse-mu-limodzi kuphatikiza nitrocellulose ndi PVDF (polyvinylidene difluoride) nembanemba.
Q: Kodi electrophoresis kusamutsa dongosolo lonse-mu-limodzi lingagwiritsidwe ntchito posanthula DNA?
A: Ayi, The electrophoresis transfer all-in-one system idapangidwa kuti iwunike mapuloteni ndipo singagwiritsidwe ntchito posanthula DNA.
Q: Ubwino wogwiritsa ntchito electrophoresis kusamutsa zonse mu umodzi dongosolo?
A: Kusamutsa kwa electrophoresis zonse-mu-kumodzi kumalola kusamutsa bwino kwa mapuloteni kuchokera ku gel kupita ku nembanemba, kumapereka kukhudzika kwakukulu komanso kutsimikizika pakuzindikira mapuloteni. Ndiwosavuta kugwiritsa ntchito njira imodzi yomwe imathandizira njira yotsekera yaku Western.
Q: Kodi electrophoresis kusamutsa dongosolo lonse-mu-modzi liyenera kusamalidwa bwanji?
A: Makina osinthira a electrophoresis amatsukidwa pakagwiritsidwa ntchito kulikonse ndikusungidwa pamalo oyera, owuma. Maelekitirodi ndi mbali zina ziyenera kuyang'aniridwa nthawi zonse ngati zowonongeka kapena zowonongeka.
