Cellulose Acetate Membrane Electrophoresis

Kodi Cellulose Acetate Membrane Electrophoresis ndi chiyani?

Cellulose Acetate Membrane Electrophoresis ndi mtundu umodzi wa njira za electrophoresis zomwe zimagwiritsa ntchito nembanemba ya cellulose acetate ngati njira yothandizira pakuyesa.

Cellulose Acetate ndi mtundu wa acetate wa cellulose womwe umachokera ku cellulose's hydroxyl.Ikasungunuka mu njira ya organic monga acetone, imatha kuphimbidwa ndi filimu yowoneka bwino komanso yowoneka bwino yokhala ndi ma pores.Makulidwe a filimuyo ndi ozungulira 0.1-0.15mm omwe ndi oyenera kuyesa.

Ubwino wa cellulose acetate nembanemba

Poyerekeza ndi pepala la fliter, ili ndi zabwino izi:

1.Zotsatira zabwino za kupatukana.Kwa zitsanzo zamapuloteni, nembanemba ya cellulose acetate imatenga ochepa aiwo, pambuyo pa utoto, imatha kutulutsa mtundu wakumbuyo.Magulu a utoto ndi omveka bwino, kotero amalimbikitsa kulondola kwa kutsimikiza kwa kuchuluka.
2.Fast ndi kusunga nthawi.Ma cellulose acetate nembanemba sakhala ndi hydyophilic kuposa pepala la fliter, motero magwiridwe antchito a electro-osmosis amakhala ochepa kuposa pepala la fliter, ndipo mphamvu yamagetsi imayendetsedwa makamaka ndi zitsanzo zomwe zimatha kufupikitsa nthawi yolekanitsa komanso kuchepetsa nthawi ya electrophoresis.Nthawi zambiri, nthawi ya cellulose acetate membrane electrophoresis ndi pafupifupi 45-60minutes.Njira yonse ya electrophoresis kuphatikiza utoto ndi utoto umangofunika mphindi 90.
3.Kukhudzidwa kwakukulu ndi zitsanzo zochepa zomwe zingagwiritsidwe ntchito.Zimangofunika 2 μL seramu mu seramu protein electrophoresis.
4.Wide ntchito.Zina za mapuloteni si zophweka kupatukana pa pepala electrophoresis, monga Alpha-fetoprotein, bacteriolytic enzyme, insulini, ndi histone etc. Koma ndi bwino ntchito mapadi acetate nembanemba electrophoresis kuwalekanitsa.
5.Easy kusunga ndi quantification.Pambuyo popaka utoto wa cellulose acetate nembanemba electrophoresis zitsanzo, kumiza zitsanzo mu njira yosakanikirana ya glacial acetic acid ndi Mowa kuti mupange mbale yowuma yowonekera yomwe imasungidwa kwa nthawi yayitali.

Chifukwa cellulose acetate nembanemba electrophoresis n'zosavuta ntchito, tsopano chimagwiritsidwa ntchito kusanthula ndi kuyesa plasma mapuloteni, lipoprotein, glycoprotein, Alpha-fetoprotein, puloteni, polypeptide, nucleic asidi ndi biomacromolecule zina.

Kukonzekera kwa Cellulose Acetate Membrane Electrophoresis

Zida zomwe zimagwiritsidwa ntchito zimaphatikizapo magetsi otsika kwambiri, thanki ya electrophoresis yopingasa.Chothandizira chothandizira ndi cellulose acetate membrane.

Ma reagents:

1.pH8.6 barbitol buffer solution: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain:Ponceau S, Trichloroacetic;
3.TBS / T kapena PBS / T: 95% ethanol, glacial acetic acid;
4.Kuyeretsa Njira: ethanol anhydrous, glacial acetic acid.

Beijing Liuyi Biotechnology Co., Ltd ili ndi makina okhwima a cellulose acetate membrane electrophoresis.Mtundu wa DYCP-38C ndi chipinda chofewa cha pepala ndi slide electrophoresis, ndi cellulose acetate membrane electrophoresis.The waukulu thupi kuumbidwa, palibe kutayikira chodabwitsa.Mtundu wamagetsi wa DYY-2C, DYY-6C, DYY-6D, DYY-8C , DYY-10C ndi DYY-12 ukhoza kupereka mphamvu kwa DYCP-38C.Nthawi zambiri, kasitomala amakonda kusankha mtundu wa DYY-6C ngati magetsi a DYCP-38C.

Monga chinthu chofunikira pa tanki ya DYCP-38C electrophoresis, Liuyi amaperekanso nembanemba ya cellulose acetate.Pali mitundu itatu yofanana ya nembanemba, 7 * 9cm, 2 * 8cm ndi 12 * 8cm, ndipo titha kupereka makonda a cellulose acetate nembanemba ngati zomwe mukufuna.

Ndemanga za kuyesa:

1.Lowani chitsanzo pa mbali yovuta: 1-2UL ndi yoyenera
2.Ma electrophoresis nthawi zonse: mphamvu yapano 0.4 ~ 0.6m A/cm
3.pH8.6 barbitol buffer yankho: Ionic mphamvu 0.06
4.Kupaka utoto: Mphindi 5 zokwanira
5.Kusungirako: ikani mapu owuma a elecrtophoresis mu njira yoyeretsera kwa mphindi 10-15, ndiyeno mutulutse ndikuyiyika pa galasi loyera, ikauma, idzakhala mapu a filimu yowonekera.

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