Agaananyamukagel electrophoresis
Agarose gel electrophoresis ndi njira ya gel electrophoresis yomwe imagwiritsidwa ntchito mu biochemistry, molecular biology, genetics, ndi chemistry yachipatala kuti alekanitse anthu osakanikirana a macromolecules monga DNA kapenaRNA.Izi zimatheka posuntha mamolekyu a nucleic acid omwe ali ndi vuto loyipa kudzera mu matrix a agaroseelectrophoresis.
Katundu wa gel osakaniza agarose
Gelisi ya Agarose ndi mawonekedwe atatu amtundu wopangidwa ndi mamolekyu a helical agarose mumitolo yapamwamba kwambiri yomwe imaphatikizidwa m'magulu atatu-dimensional ndi njira ndi pores momwe ma biomolecules amatha kudutsamo. potenthetsa kubwerera ku mkhalidwe wamadzimadzi.Kutentha kosungunuka kumasiyana ndi kutentha kwa gelling, kutengera komwe kumachokera, gel osakaniza agarose amakhala ndi kutentha kwa 35.-42 ° C ndi kutentha kwa 85-95 °C.Ma agaroses osungunuka pang'ono komanso otsika kwambiri opangidwa kudzera mukusintha kwamankhwala amapezekanso.
Ubwino ndi kuipa kwa agarose gel electrophoresis
Phindu lalikulu la njira ya agarose gel electrophoresis ndikuti imatha kukonzedwa mosavuta ndipo molekyulu ya DNA yomwe imagwiritsidwa ntchito ngati chitsanzo imatha kubwezeretsedwanso popanda kuvulaza pamapeto pake.Chifukwa agarose gel osakaniza'ilibe sulphate ndi carboxyl gulu, sizingayambitse electroosmosis ya agar.Ndi mawonekedwe a yunifolomu ndi kukula kwakukulu kwa pore, gel agarose ndi abwinokulekanitsa mamolekyu akuluakulu a DNA, monga enzyme complex, nucleic acid ndi virus.Agarose gel osakaniza't kuyatsa kuwala kwa UV, kotero imatha kuyipitsidwa mwachindunji kapena itatha gelling kukhala filimu, imasinthidwanso ndi thermally.
Palinso zovuta zina za njira ya agarose gel electrophoresis.Gelisi ya Agarose imasweka mosavuta, kotero kachulukidwe kake sikungakhale kotsika kwambiri, kumawononga mtengo wa agarose.Gel agarose n'kovuta kusunga chifukwa n'zosavuta kuipitsidwa ndi mabakiteriya, ndipo ayenera kukonzekera asanagwiritse ntchito.Poyerekeza ndi njira ya PAGE electrophoresis, mphamvu ya sieve ya maselo ndi yochepa, ndipo electrophoresis zone ndi zochepa.
Chopingasaelectrophoresisdongosolo la agarose gel
Kupatula agarose ndi buffer, kuyesa kwa agarose gel electrophoresis kumafunika njira yopingasa ya electrophoresis komanso yotsika kwambiri.magetsi electrophoresis magetsi.Beijing Liuyi Biotechnology akhoza kupereka yopingasa electrophoresis dongosolo ndi electrophoresis magetsi, monga chitsanzoChithunzi cha DYCP-31BN, Chithunzi cha DYCP-31CN zahorizontal electrophoresis dongosolo ndiDYY-6C kwa magetsi.
Kutengera pamitundu yosiyanasiyana ya gelzofunika ndi Kuyesa kwa agrose gel electrophoresis, makulidwe osiyanasiyana opingasa electrophoresis dongosolo angaperekedwe ndi Beijing Liuyi Biotechnology.Mtundu wa DYCP-31 mndandanda,sukh ngatiChithunzi cha DYCP-31BN, Chithunzi cha DYCP-31CN, Chithunzi cha DYCP-31DN, DYCP-31E, DYCP-32B,ndiDYCP-32Ckukhala ndi makulidwe osiyanasiyana a thireyi ya gel, kuyambira pakuponyera gel osakaniza mpaka gel wamkulu, amatha kukumana ndi kuyesa kwanuzofunika.
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Nthawi yotumiza: Apr-16-2022