Protein Electrophoresis Common Issues

Mapuloteni a electrophoresis band amatanthauza zovuta kapena zolakwika zomwe zingabwere panthawi yolekanitsa mapuloteni kutengera mphamvu yawo yamagetsi pogwiritsa ntchito electrophoresis.Nkhanizi zingaphatikizepo maonekedwe a magulu osayembekezeka kapena osadziwika bwino, kusasunthika bwino, kudzoza, kapena kusokonezeka kwa magulu a mapuloteni, pakati pa ena.Timapereka chidule cha zinthu zingapo zodziwika kuti makasitomala athu adziwe zifukwa zake ndikupeza zotsatira zolondola ndikuwongolera kulekanitsa kwa mapuloteni.

Kumwetuliragulu- mawonekedwe a bandi amapindikira m'mwamba mbali zonse za gel

Chifukwa
1. Pakati pa gel osakaniza akuthamanga kwambiri kuposa mapeto onse
2. Mphamvu zinthu mochulukira

Yankho
① Chotchinga chosasakanizika bwino kapena chotchingira mchipinda chapamwamba chokhazikika kwambiri.Panganinso buffer, kuwonetsetsa kusakanikirana bwino, makamaka pakuchepetsa 5x kapena 10x stock
② Chepetsani kuyika kwa magetsi kuchokera pa 200 V mpaka 150 V kapena mudzaze chipinda chocheperako mpaka 1 cm pamwamba pa mbale yayifupi.

Kuchuluka kwa mapuloteni

Chifukwa
1. Zitsanzo zadzaza
2. Chitsanzo cha mvula Yankho

Yankho
① Sungunulani zitsanzo, kusankha chotsani mapuloteni ambiri mu zitsanzo, kapena kuchepetsa mphamvu pafupifupi 25% kuti muchepetse kusuntha
② Zitsanzo za Centrifuge musanawonjezeko zachitsanzo za SDS, kapena kuchepetsa %T ya gel osakaniza
③ Chiyerekezo cha SDS ndi mapuloteni chiyenera kukhala chokwanira kuvala molekyulu ya protein iliyonse ndi SDS, nthawi zambiri 1.4: 1.Zitha kufunikira ma SDS ochulukirapo pamapuloteni a nembanemba

Bndimopingasakufalikira

Chifukwa
1. Kufalikira kwa zitsime musanayambe kuyatsa magetsi
2. Mphamvu ya Ionic ya chitsanzo chotsika kuposa ya gel

Yankho
① Chepetsani nthawi pakati pa kugwiritsa ntchito zitsanzo ndikuyatsa kuyatsa magetsi
② Gwiritsani ntchito zotchingira zomwezo muzatsanzo monga mu gelisi kapena gel osanjikiza

Magulu a mapuloteni opotoka kapena opotozedwa

Chifukwa
1. Polima polima mozungulira zitsime
2. Mchere mu chitsanzo
3. Osafanana gel osakaniza mawonekedwe

Yankho
① Degas stacking gel osakaniza njira kwathunthu pamaso kuponyera;onjezerani ammonium persulfate ndi TEMED ndende ndi 25%, pomanga gel osakaniza kapena otsika % T, siyani APS mofanana ndi kuwirikiza ndende ya TEMED.
② Chotsani mcherewo ndi dialysis, desalting;
③ Chepetsani kuchuluka kwa polymerization.Ikani ma gels mosamala kwambiri.

Beijing Liuyi Biotechnology Company Ltd imapanga zinthu zingapo zama electrophoresis zomwe zingathandize kuthana ndi zovuta zomwe mwina timakumana nazo pakuyesa kwathu kwa electrophoresis.

Beijing Liuyi Biotechnology Company Ltd yomwe idakhazikitsidwa mu 1970, yomwe kale inkadziwika kuti Beijing Liuyi Instrument Factory, ndi opanga zida za labotale ndi zida zasayansi zozikidwa ku Beijing, China.Ndili ndi zaka zopitilira 50 pakupanga ndi kupanga zinthu za electrophoresis ndi kafukufuku wa sayansi ya moyo, imakhala yopereka zida za labotale ndi mayankho ku China.Zogulitsa zamakampani zimaphatikizapo zida za labotale, kuphatikiza thanki yopingasa ya nucleic acid electrophoresis, mapuloteni osunthika. electrophoresis tank/unit, black-box type UV analyzer, Gel Document Tracking Imaging Analyzer, ndi electrophoresis magetsi.Zogulitsazi zimagwiritsidwa ntchito kwambiri m'mabungwe ofufuza, mayunivesite, ndi mafakitale osiyanasiyana, monga mankhwala, biotechnology, ndi kuyang'anira zachilengedwe. Kampaniyi ndi ISO9001 & ISO13485 kampani yotsimikizika ndipo ili ndi ziphaso za CE.

Palizosiyanasiyanaofukulaakasinja electrophoresis kwamapuloteni electrophoresiskusanthula ndi kuzindikira zitsanzo zamapuloteni ndi polyacrylamide gel electrophoresis,ndikomanso kuyeza zitsanzo za kulemera kwa maselo, kuyeretsa zitsanzo ndi kukonzekera zitsanzo.Zogulitsa izi zonse zimalandilidwamsika wapakhomo ndi wakunja.

Mphamvu yamagetsi ya electrophoresis ndi gawo lofunikira laelectrophoresis dongosolo, kupereka gwero lokhazikika komanso lolondola la magetsi kuti ayendetse njira yolekanitsa.ItNthawi zambiri amapereka magetsi okhazikika kapena okhazikika ku dongosolo la electrophoresis, kutengera ndondomeko yoyesera.Zimathandizanso wogwiritsa ntchito kusintha mphamvu yamagetsi kapena zomwe zikuchitika panopa, komanso magawo ena monga nthawi ndi kutentha, kuti athetseretu mikhalidwe yolekanitsa pakuyesera kwina.

Fkapena kuyang'ana gel osakaniza, mutha kusankha UV Transilluminator WD-9403 mndandanda wopangidwa ndi Beijing Liuyi Biotechnology.A UV transilluminator ndi chida cha labotale chomwe chimagwiritsidwa ntchito powonera ndikusanthula DNA, RNA, ndi zitsanzo zama protein.Zimagwira ntchito powunikira zitsanzo ndi kuwala kwa UV, zomwe zimapangitsa kuti zitsanzozo ziwonekere ndikuwoneka.Pali mitundu ingapo ya UV transilluminatorzoperekedwa kwa inu ndi ife.WD-9403A ndiyowona makamaka poyang'ana protein electrophoresis, ndipo WD-9403F imagwiritsidwa ntchito powonera DNA ndi protein electrophoresis.

Zogulitsa izi zitha kukhala kuchokera pakuponyera gel mpaka kuyang'ana gel molingana ndi zomwe mumayesa.Tiuzeni zomwe mukufuna, OEM, ODM ndi ogulitsa amalandiridwa.We tidzayesetsa momwe tingathere kukupatsani katundu wathu ndi ntchito.

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