Mfundo zingapo Ziyenera Kukumbukiridwa Mukamagwiritsa Ntchito Cellulose Acetate Membrane Electrophoresis (1)

Rzofunikira ofkupanga membrane:

Nembanemba yomwe imagwiritsidwa ntchito pa cellulose acetate electrophoresis imakhala ndi zofunika kwambiri. Choyamba, zopangira zopangira membrane, cellulose acetate, ziyenera kukhala zoyera kwambiri. Siyenera kukhala ndi zonyansa monga hemicellulose, lignin, kapena ma ion heavy metal. Izi ndizofunikira makamaka pakulekanitsa ndi kusanthula zitsanzo, popeza kupeza zotsatira zabwino zoyeserera kungakhale kovuta popanda nembanemba yoyera. Kachiwiri, nembanembayo iyenera kukhala ndi zokutira zofananira, makulidwe oyenera, komanso kuyamwa bwino kwamadzi. Kuti izi zitheke, nembanembayo iyenera kuphimbidwa mbali imodzi, kupewa kukwapula mobwerezabwereza. Kukula kovomerezeka kwa nembanemba kumakhala pakati pa 0.1 mpaka 0.15 mm. Ngati nembanembayo ndi yokhuthala kwambiri, imayamwa bwino madzi ndipo izi zimapangitsa kuti pasakhale kulekana. Kumbali ina, ngati nembanemba ndi woonda kwambiri, amakhala sachedwa kusweka ndipo alibe zofunika makina mphamvu.

Kuchiza kwa nembanemba pamaso pa electrophoresis:

Monga tanena kale, nembanemba ya cellulose acetate ili ndi mgwirizano wocheperako wamadzi poyerekeza ndi pepala. Chifukwa chake, ndikofunikira kuwonetsetsa kuti nembanembayo ili ndi yankho linalake la buffer kalekuthamangaelectrophoresis. Nembanemba iyenera kuviikidwa kale mu yankho la buffer. Njira yoyenera yonyowetsera nembanemba ndikuyiyandamitsa pang'onopang'ono pamwamba pa yankho la bafa, kulola nembanembayo kuti itenge yankho la bafa kuchokera pansi ndikumira mu yankho.kupewa thovu pamwamba pa nembanemba kukhudzazotsatira zolekanitsa.

Kuonjezera apo, pambuyo pa kunyezimira kwa membrane,zowonjezeraNjira yothetsera buffer iyenera kuchotsedwa pang'onopang'ono pogwiritsa ntchito pepala losefera. Siziyenera kuumitsa kwathunthu kapena kunyowa kwambiri. Ngati ndi yowuma kwambiri, imalepheretsa kupatukana kwa electrophoretic. Ngati ili yonyowa kwambiri, imatha kukhudza kutsitsa kwachitsanzo, kupangitsa kuti mizere yachitsanzo ifalikire komanso kuti zoyambira za gawo lililonse zikhale zosagwirizana, zomwe zimasokoneza magwiridwe antchito olekanitsa.

Tidzalemba zambiri za nkhaniyi nthawi ina, ndipo landirani ndemanga zanu!

Zambiri zokhudza Beijing Liuyi Biotechnology's cellulose acetate membranethanki ya electrophoresis ndi ntchito yake ya electrophoresis, chonde pitani Pano:

lKuyesa kulekanitsa mapuloteni a seramu ndi Cellulose Acetate Membrane

lCellulose Acetate Membrane Electrophoresis

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Zolozera:Electrophoresis(Kusindikiza kwachiwiri) ndi Bambo He ndi Mr.Zhang