Tinagawana zinthu zingapo sabata yatha pakugwiritsa ntchito cellulose acetate membrane electrophoresis, ndipo timaliza nkhaniyi pano lero kuti mufotokozere.
Kusankhidwa kwa Buffer Concentration
Kuchuluka kwa buffer komwe kumagwiritsidwa ntchito mu cellulose acetate membrane electrophoresis nthawi zambiri kumakhala kotsika kuposa komwe kumagwiritsidwa ntchito pama electrophoresis a pepala. Nthawi zambiri amagwiritsidwa ntchito pH 8.6Barbital buffer nthawi zambiri amasankhidwa mkati mwa 0.05 mol/L mpaka 0.09 mol/L. Posankha ndende, kutsimikiza koyambirira kumapangidwa. Mwachitsanzo, ngati kutalika kwa nembanemba Mzere pakati maelekitirodi mu electrophoresis chipinda ndi 8-10cm, voteji 25V chofunika pa centimita kutalika nembanemba, ndi mphamvu panopa ayenera kukhala 0.4-0.5 mA pa centimita m'lifupi nembanemba. Ngati izi sizikukwaniritsidwa kapena kupyola panthawi ya electrophoresis, ndende ya buffer iyenera kuwonjezeka kapena kuchepetsedwa.
Kutsika kwambiri kwa bafa kumapangitsa kuti magulu azisuntha mwachangu komanso kukula kwa bandi. Kumbali inayi, kuchuluka kwa bafa kwakukulu kumachepetsa kusamuka kwa magulu, zomwe zimapangitsa kuti zikhale zovuta kusiyanitsa magulu ena olekanitsa.
Tiyenera kuzindikira kuti mu cellulose acetate membrane electrophoresis, gawo lalikulu lamakono limachitika kudzera mu chitsanzo, chomwe chimapanga kutentha kwakukulu. Nthawi zina, ndende yosankhidwa ya buffer imatha kuonedwa ngati yoyenera. Komabe, pakuwonjezeka kwa kutentha kwa chilengedwe kapena mukamagwiritsa ntchito magetsi okwera kwambiri, kutuluka kwa madzi chifukwa cha kutentha kumatha kukulirakulira, zomwe zimapangitsa kuti pakhale kuchuluka kwamphamvu kwambiri komanso kuchititsa kuti nembanembayo iume.
Chitsanzo cha Voliyumu
Mu cellulose acetate membrane electrophoresis, kuchuluka kwa zitsanzo kumatsimikiziridwa ndi zinthu zosiyanasiyana, kuphatikiza mikhalidwe ya electrophoresis, mawonekedwe a chitsanzocho, njira zodetsa, ndi njira zodziwira. Monga mfundo yodziwika bwino, njira yodziwira yomwe imakhala yovuta kwambiri, mphamvu yachitsanzo imakhala yochepa kwambiri, yomwe imakhala yopindulitsa pakupatukana. Ngati voliyumu yachitsanzo ndiyochulukira, njira zolekanitsa ma electrophoretic sizingakhale zomveka bwino, komanso kudetsa kumathanso kutenga nthawi. Komabe, posanthula mochulukira magulu otalikirana olekanitsidwa pogwiritsa ntchito njira zodziwira zamtundu wa elution colorimetric, voliyumu yachitsanzo siyenera kukhala yaying'ono kwambiri, chifukwa imatha kupangitsa kuti zigawo zina zizikhala zocheperako, zomwe zimapangitsa kuti pakhale zolakwika zambiri pakuwerengera zomwe zili. Zikatero, kuchuluka kwachitsanzo kuyenera kuwonjezeredwa moyenerera.
Kawirikawiri, voliyumu yachitsanzo yowonjezeredwa pa centimita iliyonse ya mzere wogwiritsira ntchito chitsanzo imachokera ku 0.1 mpaka 5 μL, yofanana ndi chiwerengero cha 5 mpaka 1000 μg. Mwachitsanzo, pakuwunika kwanthawi zonse kwa mapuloteni a seramu a electrophoresis, kuchuluka kwachitsanzo komwe kumawonjezeredwa pa centimita iliyonse ya mzere wogwiritsa ntchito nthawi zambiri sikudutsa 1 μL, yofanana ndi 60 mpaka 80 μg ya mapuloteni. Komabe, posanthula ma lipoproteins kapena glycoprotein pogwiritsa ntchito njira yofananira ya electrophoresis, kuchuluka kwachitsanzo kuyenera kukulitsidwa molingana.
Pomaliza, voliyumu yachitsanzo yoyenera kwambiri iyenera kusankhidwa potengera mikhalidwe inayake kudzera muzoyeserera zoyambira.
Kusankhidwa kwa Staining Solution
Magulu opatukana mu cellulose acetate membrane electrophoresis nthawi zambiri amakhala odetsedwa asanazindikiridwe. Zigawo zosiyanasiyana zachitsanzo zimafuna njira zosiyanasiyana zodetsa, ndipo njira zodetsa zoyenera pa cellulose acetate membrane electrophoresis sizingakhale zogwira ntchito pakusefa pepala.
Pali mfundo zazikulu zitatu zomwe mungasankhe njira yothetsera madonthocellulose acetate membrane. Choyamba,Utoto wosungunuka m'madzi uyenera kukhala wokondeka kuposa utoto wosungunuka moŵa kuti upewe kuchepa kwa nembanemba ndi kupindika komwe kumachitika chifukwa cha utoto wothimbirira. Pambuyo pakudetsa, ndikofunikira kutsuka nembanembayo ndi madzi ndikuchepetsa nthawi yodetsa. Kupanda kutero, nembanemba imatha kupindika kapena kufota, zomwe zingakhudze kuzindikirika.
Kachiwiri, ndikwabwino kusankha utoto wokhala ndi madontho amphamvu amtundu wa chitsanzo. Mu cellulose acetate membrane electrophoresis ya mapuloteni a seramu, amino wakuda 10B amagwiritsidwa ntchito nthawi zambiri chifukwa cha kuyanjana kwake kodetsa kolimba kwa zigawo zingapo zamapuloteni a seramu komanso kukhazikika kwake.
Chachitatu, utoto wodalirika uyenera kusankhidwa. Utoto wina, ngakhale uli ndi dzina lomwelo, ukhoza kukhala ndi zonyansa zomwe zimapangitsa kuti pakhale mdima wakuda pambuyo poyipa. Izi zimatha kusokoneza magulu omwe anali olekanitsidwa bwino, zomwe zimapangitsa kuti zikhale zovuta kuzisiyanitsa.
Pomaliza, kusankha ma staining solution ndende ndikofunikira. Mwachidziwitso, zitha kuwoneka kuti kuyika kwa ma staining solution kungayambitse kudetsa bwino kwa zigawo zachitsanzo ndi zotsatira zabwino zodetsa. Komabe, izi sizili choncho. Kugwirizana komangiriza pakati pa zigawo zachitsanzo ndi utoto kumakhala ndi malire, omwe samawonjezeka ndi kuwonjezereka kwa ndende yazitsulo zowononga. M'malo mwake, kupaka utoto wochuluka kwambiri sikungowononga utoto komanso kumapangitsa kuti zikhale zovuta kupeza maziko omveka bwino. Komanso, mphamvu ya utoto ikafika pamlingo wina wake, piritsi la utoto silimayenderana ndi mzere, makamaka poyeza kuchuluka kwake.
Zambiri zokhudza Beijing Liuyi Biotechnology's cellulose acetate membranethanki ya electrophoresis ndi ntchito yake ya electrophoresis, chonde pitani Pano:
lKuyesa kulekanitsa mapuloteni a seramu ndi Cellulose Acetate Membrane
lCellulose Acetate Membrane Electrophoresis
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Zolozera:Electrophoresis(Kusindikiza kwachiwiri) ndi Bambo Li
Nthawi yotumiza: Jun-06-2023