DYCP-40C semi-dry blotting system imagwiritsidwa ntchito limodzi ndi magetsi a electrophoresis posamutsa mapuloteni a polyacrylamide gels pa nembanemba ngati nembanemba ya nitrocellulose, nembanemba ya nayiloni ndi nembanemba ya PVDF.Semi-dry blotting imachitidwa ndi maelekitirodi a graphite mbale mopingasa, ndikuyika gel osakaniza ndi nembanemba pakati pa mapepala a fyuluta oviikidwa m'miyendo yomwe imagwira ntchito ngati nkhokwe ya ion.Pamene electrophoretic kusamutsidwa, mamolekyu oipa mlandu amachoka mu gel osakaniza ndi kupita ku zabwino electrode, kumene waikamo pa nembanemba.Maelekitirodi a mbale, olekanitsidwa ndi gel osakaniza ndi mapepala osefera, amapereka mphamvu yamtunda (V / cm) kudutsa gel osakaniza, kupititsa patsogolo kwambiri, mofulumira.Malo osinthira ang'onoang'ono a DYCP - 40C electrophoresis cell ndi 150 × 150 (mm), oyenera kusamutsa ma gels wamba, kuphatikiza omwe amachokera ku DYCZ-24DN ndi DYCZ-24EN electrophoresis cell.
Tiyeni tiphunzire zambiri za kagwiritsidwe ntchito ka zida za semi-dry trans blot.
Zida, zida zogwiritsira ntchito DYCP-40C
Magetsi a Electrophoresis DYY-6C, zida za semi-dry trans blot DYCP-40C, yankho la buffer, ndi zotengera zopangira yankho.ndi zina.
Njira Zogwirira Ntchito
1. Ikani gel osakaniza ndi mbale zagalasi mu njira yosinthira yotchinga
2. Yezerani kukula kwa gel osakaniza
3.Konzani zidutswa zitatu za pepala losefera molingana ndi kukula kwa gel osakaniza, ndipo kukula kwa pepala losefera kuyenera kukhala kokulirapo kuposa kukula kwa gelsi;Apa timagwiritsa ntchito pepala losefera la Whatman;
4.Ikani zidutswa zitatu za pepala losefera mu njira yotchingira pang'onopang'ono, ndipo lolani pepala losefera kumizidwa kwathunthu mu nkhokwe, ndipo pewani kukhala ndi thovu la mpweya;
5.Konzani ndikudula nembanemba ya nitrocellulose molingana ndi kukula kwa gel osakaniza ndi pepala losefera;kukula kwa nitrocellulose nembanemba ayenera kukhala wamkulu kuposa gel osakaniza ndi fyuluta pepala;
6.Ikani nembanemba ya nitrocellulose mu yankho la bafa;
7.Chotsani zidutswa zitatu zosefera pepala ndikuponya njira yowonjezera yotchingira mpaka yankho la bafa lisanagwe pa nembanemba;ndiyeno ikani pepala losefera pansi pa DYCP-40C;
8.Tengani gel osakaniza kuchokera m'mbale zagalasi, yeretsani gel osakaniza pang'onopang'ono, ndikuyika gel osakaniza mu njira yothetsera;
9 .Ikani gel osakaniza pa pepala fyuluta, kuyambira kumapeto kwa gel osakaniza kupewa thovu mpweya;
10.Gwiritsani ntchito chida choyenera kuchotsa thovu la mpweya pakati pa gel osakaniza ndi pepala losefera.
11.Phimbani nembanemba ya nitrocellulose pa gel, mbali yoyipa yoyang'ana gel.Kenako gwiritsani ntchito chida choyenera kuti muchotse thovu la mpweya pakati pa nembanemba ndi gel.Ikani zidutswa zitatu za pepala losefera pa nembanemba.Muyeneranso kugwiritsa ntchito chida choyenera kuchotsa thovu la mpweya pakati pa pepala losefera ndi nembanemba.
12.Phimbani chivindikirocho, ndikuyika magawo a electrophoresis, 80mA yokhazikika;
13.Electrophoresis yachitika.Timapeza zotsatira monga zotsatirazi;
Beijing Liuyi Biotechnology Co., Ltd yakhala ikupanga zinthu za electrophoresis kwazaka zopitilira 50.Ndife ISO9001 & ISO13485 kampani yotsimikizika komanso okhazikika pakupanga akasinja a electrophoresis, zida zamagetsi, UV transilluminator, ndi zolemba za gel & kusanthula dongosolo, pakadali pano, timapereka chithandizo cha OEM kwa makasitomala athu, komanso ntchito ya ODM.
Tsopano tikuyang'ana mabwenzi, onse OEM ndi ogulitsa amalandiridwa.
Ngati muli ndi pulani yogulira zinthu zathu, chonde musazengereze kulumikizana nafe.Mutha kutitumizira uthenga pa imelo[imelo yotetezedwa]kapena[imelo yotetezedwa], kapena chonde tiyimbireni ku +86 15810650221 kapena onjezani Whatsapp +86 15810650221, kapena Wechat: 15810650221.
Nthawi yotumiza: Apr-04-2023