PCR Thermal Cycler WD-9402D
Zofotokozera
| Chitsanzo | WD-9402D |
| Mphamvu | 96 × 0.2ml |
| Chubu | 0.2ml chubu, 8 n'kupanga, Hafu skirt96 zitsime mbale, Palibe siketi 96 zitsime mbale |
| Rection Volume | 5-100 l |
| Kutentha Kusiyanasiyana | 0-105 ℃ |
| MAX.Ramp Rate | 5℃/s |
| Kufanana | ≤± 0.2 ℃ |
| Kulondola | ≤±0.1℃ |
| Kuwonetseratu | 0.1 ℃ |
| Kuwongolera Kutentha | Block/Tube |
| Ramping Rate Kusintha | 0.01-5 ℃ |
| Mtengo wa Gradient.Mtundu | 30-105 ℃ |
| Mtundu wa Gradient | Normal Gradient |
| Kufalikira kwa Gradient | 1-42 ℃ |
| Kutentha kwa Lid | 30-115 ℃ |
| Chiwerengero cha Mapulogalamu | 20000 + (USB FLASH) |
| Max.Nambala ya Step | 40 |
| Max.No. ya Cycle | 200 |
| Kuwonjezeka kwa Nthawi / Kuchepetsa | 1 Sec - 600 Sec |
| Kutentha Kuwonjezeka/Kuchepa | 0.1-10.0 ℃ |
| Imani Ntchito | Inde |
| Chitetezo cha Auto Data | Inde |
| Gwirani pa 4 ℃ | Kwamuyaya |
| Touchdown Ntchito | Inde |
| Ntchito yayitali ya PCR | Inde |
| Chiyankhulo | Chingerezi |
| Mapulogalamu apakompyuta | Inde |
| Foni yam'manja APP | Inde |
| LCD | 10.1 inchi, 1280 × 800 pellets |
| Kulankhulana | USB2.0, WIFI |
| Makulidwe | 385mm × 270mm × 255mm (L×W×H) |
| Kulemera | 10kg pa |
| Magetsi | 100-240VAC , 50/60Hz , 600 W |
Kufotokozera
Thermal cycler imagwira ntchito potenthetsa mobwerezabwereza ndikuziziritsa zomwe zili ndi DNA kapena RNA template, primers, ndi nucleotides.Kutentha kwapang'onopang'ono kumawongoleredwa bwino kuti mukwaniritse zofunikira, kuyika, ndi kukulitsa njira ya PCR.
Nthawi zambiri, woyendetsa matenthedwe amakhala ndi chipika chokhala ndi zitsime zingapo kapena machubu pomwe chosakanizacho chimayikidwa, ndipo kutentha kwa chitsime chilichonse kumayendetsedwa palokha.Chotchingacho chimatenthedwa ndikukhazikika pogwiritsa ntchito chinthu cha Peltier kapena makina ena otenthetsera ndi ozizira.
Ma cyclers ambiri otentha amakhala ndi mawonekedwe osavuta kugwiritsa ntchito omwe amalola wogwiritsa ntchito kukonza ndikusintha magawo apanjinga, monga kutentha kwa annealing, nthawi yowonjezera, ndi kuchuluka kwa ma cycle.Athanso kukhala ndi chiwonetsero chowonera momwe zimachitikira, ndipo mitundu ina imatha kupereka zinthu zapamwamba monga kuwongolera kutentha kwa gradient, masinthidwe angapo a block, ndi kuyang'anira ndi kuyang'anira kutali.
Kugwiritsa ntchito
Kupanga ma genome;Kukonzekera kwa Asymmetric PCR kwa DNA yokhala ndi chingwe chimodzi pakutsata kwa DNA;Inverse PCR kuti mudziwe madera osadziwika a DNA;reverse transcription PCR (RT-PCR).Kuzindikira mulingo wa jini m'maselo, ndi kuchuluka kwa kachilombo ka RNA ndikupangidwa mwachindunji kwa cDNA yokhala ndi majini enieni;kukulitsa mwachangu malekezero a cDNA;kuzindikira kwa jini;angagwiritsidwe ntchito kudziwika kwa bakiteriya ndi tizilombo matenda;matenda a majini;matenda a zotupa;kafukufuku wamankhwala monga umboni wazamalamulo, sungagwiritsidwe ntchito pofufuza zamankhwala.
Zowonetsedwa
• Kutentha kwakukulu ndi kuzizira, max.Ramping mlingo 8 ℃/s;
• Yambitsaninso zokha mukatha mphamvu.Mphamvu ikabwezeretsedwa imatha kupitiliza kuyendetsa pulogalamu yosamalizidwa;
• Kudina kamodzi kofulumira kwa makulitsidwe kungakwaniritse zoyeserera monga denaturation, enzyme cutting/enzyme-link ndi ELISA;
• Kutentha kwa chivindikiro chotentha ndi ntchito ya chivindikiro yotentha ikhoza kukhazikitsidwa kuti ikwaniritse zosowa zosiyanasiyana;
• Amagwiritsa ntchito ma module a Peltier a moyo wautali wa kutentha kwapang'onopang'ono;
• Anodized aluminiyamu module ndi kulimbikitsa zomangamanga, amene amasunga mofulumira kutentha conduction ntchito ndipo ali ndi kukana dzimbiri zokwanira;
• Kutentha kwachangu, ndi mlingo wapamwamba wa 5 ° C / s, kupulumutsa nthawi yoyesera yofunikira;
• Chivundikiro chotenthetsera cha bar-style chotenthetsera, chomwe chitha kutsekedwa mwamphamvu ndi sitepe imodzi ndipo chimatha kutengera kutalika kwa chubu;
• Kukonzekera kwa mpweya wobwerera kumbuyo, kulola makina kuti aziyika mbali imodzi;
• Amagwiritsa ntchito makina ogwiritsira ntchito a Android, omwe amafanana ndi 10.1-inch capacitive touch screen, yokhala ndi mawonekedwe amtundu wa menyu, zomwe zimapangitsa kuti ntchito ikhale yosavuta kwambiri;
• Ma templates omangidwa mu 11 okhazikika, omwe amatha kusintha mwachangu mafayilo ofunikira;
• Kuwonetsa nthawi yeniyeni ya kupita patsogolo kwa pulogalamu ndi nthawi yotsalira, kuthandizira pulogalamu yapakatikati ya chida cha PCR;
• Batani limodzi lofulumira kugwira ntchito, kukwaniritsa zosowa za mayesero monga denaturation, enzyme digestion / ligation, ndi ELISA;
• Kutentha kwa chivundikiro chotentha ndi njira yogwiritsira ntchito chivundikiro chotentha ikhoza kukhazikitsidwa kuti ikwaniritse zosowa zosiyanasiyana zoyesera;
• Chitetezo chozimitsa magetsi, kuchita zokha mizere yosamalizidwa mphamvu ikabwezeretsedwa, kuwonetsetsa kuti ikugwira ntchito motetezeka panthawi yonse yokulitsa;
• Mawonekedwe a USB amathandizira kusungidwa kwa data ya PCR / kubwezeretsa pogwiritsa ntchito USB drive ndipo amathanso kugwiritsa ntchito mbewa ya USB kuwongolera chida cha PCR;
• Imathandizira zosintha zamapulogalamu kudzera pa USB ndi LAN;
• Ma module a WIFI omangidwa, kulola kompyuta kapena foni yam'manja kulamulira nthawi imodzi zida zambiri za PCR kudzera pa intaneti;
• Imathandizira zidziwitso za imelo pamene pulogalamu yoyeserera yatha.
FAQ
Q: Kodi woyendetsa matenthedwe ndi chiyani?
Yankho: Makina oyendetsa matenthedwe ndi chipangizo cha labotale chomwe chimagwiritsidwa ntchito kukulitsa ma DNA kapena ma RNA kudzera mu polymerase chain reaction (PCR).Zimagwira ntchito poyendetsa njinga kudzera mukusintha kwa kutentha, zomwe zimapangitsa kuti ma DNA atsatidwe kuti akwezedwe.
Q: Ndi zigawo ziti zazikulu za woyendetsa matenthedwe?
A: Zigawo zazikulu za cycler matenthedwe zimaphatikizapo chipika chotenthetsera, chozizira cha thermoelectric, masensa kutentha, microprocessor, ndi gulu lowongolera.
Q: Kodi chozungulira chotenthetsera chimagwira ntchito bwanji?
Yankho: Woyendetsa matenthedwe amagwira ntchito potenthetsa ndi kuziziritsa zitsanzo za DNA motsatizana za kutentha.Kupalasa njinga kumaphatikizapo ma denaturation, annealing, ndi masitepe owonjezera, chilichonse chimakhala ndi kutentha kwake komanso nthawi yake.Zozungulira izi zimalola kuti ma DNA awonjezeke kudzera mu polymerase chain reaction (PCR).
Q: Ndi zinthu ziti zofunika kuziganizira posankha choyendetsa matenthedwe?Yankho: Zina zofunika kuziganizira posankha choyendetsa chotenthetsera ndi monga kuchuluka kwa zitsime kapena machubu ochitira, kuchuluka kwa kutentha ndi liwiro la mtunda, kulondola komanso kufanana kwa kutentha, komanso mawonekedwe a wogwiritsa ntchito ndi mapulogalamu.
Q: Kodi mumasunga bwanji chozungulira chotenthetsera?
Yankho: Kuti musunge chozungulira chotenthetsera, ndikofunikira kuyeretsa chotchingira chotenthetsera nthawi zonse ndi machubu ochitirapo kanthu, kuyang'ana kung'ambika ndi kung'ambika pazigawo zake, ndikuwongolera masensa a kutentha kuti muwonetsetse kutentha kolondola komanso kosasintha.Ndikofunikiranso kutsatira malangizo a wopanga pokonza ndi kukonza nthawi zonse.
Q: Ndi njira ziti zomwe zimakonda kuthetsera vuto kwa woyendetsa matenthedwe?
Yankho: Njira zina zothanirana ndi vuto la woyendetsa matenthedwe ndi monga kuyang'ana zida zotayirira kapena zowonongeka, kutsimikizira kutentha koyenera ndi nthawi, ndikuyesa machubu kapena mbale zomwe zawonongeka kapena kuwonongeka.Ndikofunikiranso kutchula malangizo a wopanga kuti mupeze njira zothetsera mavuto ndi zothetsera.
